中国美利奴羊多胎品系选育中多胎性状分离与BMPR-IB基因型的相关性分析

Relationship Analysis of Genotype of BMPR-IB gene and Segregation of Prolific Character in Chinese Merino Prolific Strain Sheep

以绵羊BMPR-IB基因为主效基因,以中国美利奴羊(新疆军垦型)多胎品系为研究对象,应用Genopro软件绘制多胎品系绵羊的系谱,记录母羊产羔数,采用PCR-RFLP方法对BMPR-IB基因进行基因型分型,分析多胎性状的分离规律,研究BMPR-IB基因型分布与以多胎性能为目标的品系培育的相关性。结果表明,在品系培育中,BMPR-IB基因的表型符合孟德尔遗传分离模式,增加绵羊产羔数由常染色体突变所致,BMPR-IB基因可以用于对绵羊产羔数的选择。

The prolific strain of Chinese Merino （Junken Type） with recording litter size was used in this study, the study was designed to draw pedigree and detect SNPs within BMPR-IB gene. Primers for BMPR-IB gene were designed from database sheep sequence and polymorphisms were detected by PCR-RFLP method. The pedigree were drawed by Genopro software. The results showed that the distribution of litter size among progeny of Chinese Merino （Junken Type） Prolific Strain Sheep and BMPR-IB genotype were consistent with the Mendelian inheritance model. The A746G site of BMPR-IB gene was a dominant autosomal mutant with increasing litter size. The BMPR-IB gene could be used as molecular genetic marker to select litter size in sheep.