MTHBM-Ni荧光探针法快速测定氧氟沙星

Rapid Determination of Ofloxacin in Tablets with Ni(Ⅱ) Complex of MTHBM as a Fluorescence Probe

摘要以2’-（4-甲基-2-噻吩亚甲基）-3，5-二羟基苯甲酰腙镍（MTHBM-Ni）配合物作荧光探针，用荧光光度法测定了氧氟沙星（OFLX）片中OFLX的含量。实验结果表明，当底液中MTHBM-Ni的加入量为0．8mL时，OFLX的检出限为0．16ng／mL，线性范围为（0．0033～9．45）μg/mL。方法用于OFLX片剂中OFLX的测定，RSD1．4％（n=6），平均回收率为95．70％。

A fluorimetric method for the determination of ofloxacin （OFLX） is developed with the 2＇- （4-methyl- 2-thienylmethyllene）-3,5-dihydroxybenzoylhydrazide （MTHBM-Ni） as a fluorescence probe. The results showed that the linear rang of OFLX is （0.0033 - 9.45 ） μg/mL with a detection limit of 0.16 ng/mL when the amount of MTHBM-Ni is 0.8 milliliter in basic solution. The method applied to determination of ofloxacin in tablets with 1.4% RSD（n =6）. The average recovery is 95.70%.