应用PCR扩增检测伊氏锥虫的研究

DETECTION OF TRYPANOSOMA EVANSI BY PCR

本文建立了伊氏锥虫ＤＮＡ的ＰＣＲ扩增技术，并用于伊氏锥虫的检测。经ＰＣＲ扩增后的ＤＮＡ片断分子量约为２３７ｂｐ，可以检测到０．１ｐｇ总ＤＮＡ或１条虫体，可以诊断小鼠的早期感染（第一天）和重复感染，还可以用来考核抗锥虫药物疗效；该项技术对锥虫具有特异性，且灵敏性和准确性都很高。

he method of amplification of DNA is established to detect Trypanosoma evansi.The amplified fragment of DNA is 237bp,which can be used to detect 0.1pg of the whole DNA or 1 Trypanosoma evansi,to diagnosis the early infection(the first day in mice)and repeated infection,and to observe the efficiency of antitrypanosoma drug.The results show that this technique is specific for trypanosomes,highly sensitive and accurate.