食盐对草鱼血清超氧化物歧化酶和溶菌酶活性的影响

Effects of Sodium Chloride on Activities of Superoxide Dismutase and Lysozyme in Serum of Grass Carp(Ctenpharyngodon idella)

草鱼养殖过程中经常使用食盐药浴,以便在高渗环境下杀死病原体。研究食盐药浴后草鱼代谢酶变化,有助于了解食盐药浴对草鱼机体生理免疫的影响。将草鱼(Ctenpharyngodon idella)分别暴露于食盐浓度为0、2%、3%、4%水体中浸浴10min,然后置入水簇箱中饲养,分别在药浴后12h、24h、36h取样,测定其血清中超氧化物歧化酶(SOD)和溶菌酶(LSZ)活性变化。结果显示,与对照组相比,血清SOD活性在12h时,2%组中显著上升(P<0.05),3%、4%组显著下降(P<0.05);24h取样时,2%、3%组中无显著差异(P>0.05),4%组显著下降(P<0.05);36h取样时,各组间均无显著差异(P>0.05);血清LSZ活性各组间均无显著差异(P>0.05)。食盐能引起草鱼SOD活性变化,而对溶菌酶活性影响不大;表明食盐对草鱼不同代谢酶有不同的反应模式,抗氧化酶SOD对食盐应激有敏感的生理应答。

Grass carp（ Ctenpharyngodon idella） is sensitive to environmental changes. As the highly concentration sodium chloride is used to kill pathogens under hyper -osmotic condition, stresses will be produced on physiological metabolism in grass carp. Superoxide dismutase and Lysozyme play an important role in regulating physiological metabolism. To study activity changes of superoxide dismutase and lysozyme in serum can help understand the effects of sodium chloride on physiological metabolism of grass carp. In this study, grass carps were randomly grouped into 4 groups （3 experimental groups and one control group, each group with 30 fish）. Experimental fishes exposed to different salt concentrations （2% ,3 % ,4% ） for 10 rain were replaced under the normal condition respectively. At Hour 12,24 and 36 respectively, serum of each fish was sampled to analyze. Compared with the control, at Hour 12, SOD activity increased significantly at 2% of sodium chloride, while decreased significantly at 3% and 4%. At Hour 24, SOD activity did not show significant change at 2% and 3%, while decreased significantly at 4%. At Hour 36, the activity of SOD presented no obvious changes at all experimental concentration. For Lysozyme activity, no significant change was shown at different times at various experimental concentrations. In summary, different metabolism enzymes in serum of grass carp show different physiological reactions to sodium chloride stress, and SOD indicates the sensitive physiological response.