不同离心方法制备富血小板血浆对血小板浓度及其活性的影响

The Effect of Different Platelet-rich Plasma Preparation Methods on Concentration and Activity of Platelet

目的:探讨不同方法制备对PRP中血小板浓度以及活性的影响。方法:从新西兰大白兔颈部静脉取血17ml,选用4种不同的离心方法制备PRP。离心方法一:一次离心力200g,二次离心力200g;离心方法二:一次离心力200g,二次离心力1200g;离心方法三:一次离心力1200g,二次离心力200g;离心方法四:一次离心力1200g,二次离心力1200g。离心时间均为10分钟。测量所得PRP中血小板计数、血小板回收率和归一化血浆P选择素浓度。结果:不同离心方法制备的PRP中,血小板计数明显高于全血。离心方法二所得的PRP回收率最高,为74.51%。归一化血浆P选择素浓度,离心方法二的最低。结论:采用离心方法二制备PRP,血小板浓度和回收率高,避免了离心过程导致的血小板聚集。该方法可能是PRP制备较有效的方法。

Objective： The purpose of the present study was to investigate the effect of different PRP preparation methods on concentration and activity of platelet. Methods： Seventeen milliliters whole blood was collected from jugular vein of the albino rabbit. PRP was prepared through twice centrifugation method. It was divided into four groups. First method is centrifugation at 200g for 10 minutes and further 200g for 10 minutes. The second one is centrifugation at 200g for 10 minutes and further 1200g for 10 minutes. The third one is eentrifugation at 1200g for 10 minutes and further 200g for 10 minutes. The last method is centrifugation at 1200g for 10 minutes and further 1200g for 10 minutes. Counting of the platelet concentration and percent recovery of platelet were performed. Concentration of P-selectin was measured and normalized by platelet counting. Results： Platelet counts in PRP prepared by four different methods were increased with respect to venous blood platelet counts. Percent recovery of platelet by the second method was observed to be significantly higher when compared to other methods. And the concentration of P-selectin normalized by platelet counts in PRP prepared by the second method was observed to be the lowest of all. Conclusion： It was concluded that PRP prepared by centrifugation at 200g for 10 minutes and further 1200g for 10 minutes has the higher platelet count and percent recovery of platelet and decrease centrifugation -induced platelet aggregation. It may be an efficient method for PRP preparation.