植物次生物质对棉铃虫谷胱甘肽S—转移酶和乙酰胆碱酯酶的影响

EFFECTS OF PLANT ALLELOCHEMICALS ON THE GLUTATHIONE -S-TRANSFERASE AND ACETYLCHOLINESTERASE IN HELICOVERPA ARMIGERA

分别用含0.01％的芸香甙、槲皮素和2—十三烷酮的人工饲料连续饲养棉铃虫Helicoverpa armigera H(?)bner 1～7代,测定不同处理的棉铃虫种群谷胱甘肽S—转移酶(GST)和乙酰胆碱酯酶(AChE)的活性变化以及幼虫对杀虫剂的反应,以单独人工饲料饲养的种群作为对照。芸香甙F_1代对甲基对硫磷的耐药性提高3倍,槲皮素F_1、F_2代和2—十三烷酮F_1代对甲基对硫磷的耐药性均有所提高,对灭多威和溴氰菊酯的耐药性则变化不大。芸香甙F_1代GST活性是对照的57倍。各处理种群GST的活性和AChE对底物的亲和力都随着棉铃虫取食世代的增加而提高。但是,AChE对对氧磷的敏感度也在不断提高。说明植物次生物质对棉铃虫耐药性的影响是解毒酶和靶标酶改变的综合结果。

Effects of plant allelochemicals on glutathione-S-transferase (GST) and acetylcholinesterase (AChE) activities were studied with midgut or head homogenates of larvae in Helicoverpa armigera Hubner that had reared on an artificial diet containing 0. 01% of rutin, quercetin or 2-tridaconone for 1 - 7 generations. The toxicity of methyl parathion to rutin-fed F1 , F3 and F4 generation was 3 times, 2 times and 2 times lower comparing with artificial diet-fed generations respectively. The activity of GST from cotton bollworm fed on the artificial diet containing allelochemicals is much higher than those fed on artificial diet. The affinity between AChE to acectylthiocholine (ATCh) and the sensitivity of AChE to paraoxon increased significantly. The effect of plant allelochemicals on cotton bollworm susceptibility to insecticides may be determined by the integrated results of the detoxifying enzymes and AChE changes.