离心运动训练对骨骼肌小热休克蛋白和骨架蛋白含量影响的研究

Research on the Effect of Centrifugal Training on the Content of Skeletal Muscle SHSP and Desmin

将大鼠分为安静对照组和离心训练3周组、离心训练6周组,之后安静对照组和训练6周组做大强度离心运动;取大鼠腓肠肌,采用蛋白免疫印迹法分析αB-晶体蛋白含量变化,采用免疫组织化学法分析骨架蛋白Desm in变化。结果:6周的间歇性离心训练使腓肠肌αB-晶状体蛋白有显著的增加,骨架蛋白Desm in丢失减少,CK显著的降低,说明增加的αB-晶状体蛋白对骨架蛋白有保护作用;而未训练大鼠在大强度离心运动中体验了一个显著的应激反应,骨架蛋白Desm in显著丢失,αB-crystallin有显著增加。结论:小热休克蛋白αB-晶状体蛋白在离心运动引起的骨架蛋白损伤中可能是一种补充保护作用机制。

Rats are divided into the control group, centrifugal the three -week centrifugal training group, and the six -week training group. Then rats in the control group and the six - week centrifugal training group are made to do intense centrifugal exercise. The gastroenemius muscle is taken out and Westem Blotting is employed to analyze the change of αB - crystallin protein content and IHC is employed to analyze the change of desmin. Results ： The six - week centrifugal training has markedly increased the content of gastrocnemius muscle and αB - crystallin protein, reduced the loss of desmin and lowered CK, which shows that the increased αB - crystallin protein has a protective effect on desmin. While the untrained rats have an obvious stress response after the intense centrifugal exercise with desmin greatly lost and αB - crystallin protein markedly increased. Conclusion： SHSP and αB - crystallin protein might be a kind of protection mechanism in desmin injuries induced by centrifugal exercise.