利用SSR荧光标记分析90个糯玉米地方品种的遗传多样性

Analysis of Genetic Diversity Among 90 Waxy Corn Landraces Using Fluorescent SSR Markers

采取混合取样策略,利用荧光SSR技术对来源于我国7个省(自治区)的90份糯玉米地方品种和6个标准测验种自交系基因组的31个简单序列重复(SSR)位点进行了分析。在90个糯玉米地方品种中,共检测出216个等位变异,每个位点检测出2～12个,平均6.97个,多态性信息量(PIC,polymorphic information content)为0.67,标记指数(MI,marker index)为4.92。可初步将90份糯玉米地方品种划为3个类群,其中,广西和贵州的糯玉米地方品种的遗传多样性相对更丰富。

Genetic diversity and genetic variation among 90 waxy corn landraces from 7 provinces of China and 6 common tester lines were analyzed with 31 fluorescent SSR loci across the maize genome by using a bulk sampling strategy. Totally, 216 alleles were detected with an average of 6.97, ranged from 2 to 12 per locus. The average poly- morphism information content （PIC） and marker index （MI） were 0.67 and 4.92, respectively. 90 waxy corn landraees were clustered into 3 groups. The results also showed that the Guangxi and Guizhou were the genetic diversity center for waxy corn germplasm.