玉米光周期敏感特性研究及长日照光周期诱导差减文库构建

Study on Characters of Photoperiod Sensitivity and Construction of SSH Library Affected by Long-day Photoperiod in Maize

以热带玉米自交系CML288为材料,通过对比研究表明,CML288对光周期非常敏感。通过长、短日照挪移试验发现,CML288短日照处理条件下,6～7叶期是光周期反应最敏感时期,7～9叶期是光周期敏感持续期,但敏感效应明显减弱。以CML288敏感期叶片与茎尖为材料,利用SSH技术构建了对长日照敏感而特异表达的基因文库,3000多个单克隆被有效富集。对差减文库中分离的64个特异表达EST序列分析表明,16个EST序列与玉米已知功能的蛋白序列具有较高的同源性;41个序列与其它物种编码序列同源性较高;7个EST没有同源的序列,可能代表了新基因。

We study CML288 in order to utilize tropical and subtropical germplasm better. The inbred lines, CML288 was very sensitive to photoperiod by comparing. It shows that the tassel difference transferred from SD to LD condition in sixth leaf period was invisible but in seventh, the tassel development between seven-leaf and eight-leaf period were later than the tassel in short days consistently. The results indicated that CML288 was more sensitive to photoperiod between sixth and seventh leaf period. A subtracted eDNA library was constructed by suppression subtractive hybridization（SSH） with the mRNA of leaf and shoot apices meristem of 7th leaf stage, it contained more than 3000 clones related to photoperiod sensitivity. We analyzed 64 clones, results showed that 16 ESTs were high homologous with maize sequences related to transcription factors, signal transduction, development, flowering, adversity-stressed, and so on. 41 ESTs were homologous with other plants genes. 7 EST sequences may be the new genes.