高盐低pH值法提取玉米基因组DNA的研究

Studies on Genomic DNA Extraction of Maize with High Salt,Low pH Method

优化建立了基于玉米叶片的高盐低pH值法提取玉米基因组DNA,经琼脂糖电泳检测表明,提取的DNA主带清晰、无降解现象、质量好,满足基于SSR标记的5色荧光电泳检测系统。此方法比目前提取植物基因组DNA常用的CTAB法和SDS法具有成本低、步骤简单、不使用酚、氯仿、异戊醇等有毒有机试剂的优点,是提取玉米基因组DNA的一种较好的方法。

A low pH extraction medium with high salts were successfully used to obtain total DNA from maize, the DNA yields, quality and purity were characterized. Low pH method with minor modification yields much more maize DNA, about 1 000 ug per gram of leaf tissue on a fresh leaf. These isolated DNA could be used directly for PCR analysis which is useful as molecular genetic markers.