C-反应蛋白对脂肪细胞脂联素表达的影响

Effects of C-reactive protein on adiponectin expressed adipocytes

通过体外培养脂肪细胞,研究C-反应蛋白(CRP)对大鼠脂肪细胞脂联素蛋白分泌及mRNA基因表达的影响。取大鼠附睾脂肪垫培养脂肪细胞。用0、10、50μg/mL的CRP刺激脂肪细胞6 h,提取细胞RNA,用实时荧光定量RT-PCR技术检测脂联素mRNA表达的变化;收集细胞培养液,运用Western blot技术检测脂联素蛋白分泌的变化。结果显示0、10、50μg/mL的CRP对大鼠脂肪细胞脂联素mRNA表达的影响无差异(P>0.05)。50、10μg/mL的CRP均可减少大鼠脂肪细胞培养液中脂联素蛋白的分泌量(P<0.05)。CRP可呈剂量依赖性的降低脂肪细胞脂联素蛋白分泌的水平。而各组CRP未能影响脂肪细胞脂联素mRNA的表达。CRP对脂肪细胞脂联素基因表达和蛋白分泌的研究可以揭示转录后的控制决定了CRP对脂联素的影响。

The adipocytes were obtained from rat epididymal fat pads and were incubated with C-reactive protein at the concentration of（CRP） 0,10 and 50ug/mL respectively for 6 hours to investigate the effects of C RP on adiponectin mRNA production and protein secretion. Then the medium of the adipocytes was collected and the secretion of adiponectin in the medium was determined using Western blot. Total RNA was extracted from adipocytes and adiponectin gene was expressed normally relative to untreated control cells. Adiponectin mRNA was measured by quantitative real-time reverse transcription-polymerase chain reaction. Results showed that the expression of adiponectin mRNA could not be regulated by C RP （0,10,50ug/mL） in rat adipocytes（P 〉0. 05）. Compared with control group ,the secretion of adiponeetin could be inhibited by CRP （10,50 ug/mL） in rat adipocytes（ P 〈 0. 05 ）. Adiponectin secretion is inhibited by CRP in a dose-dependent manner. But CRP could not regulate adiponectin mRNA expression. So the effect of CRP on adiponectin mRNA production and protein secretion suggests posttranscriptional mechanisms are determinants of regulation of CRP on adiponectin.