蒺藜提取物对ADR所致大鼠心肌线粒体损伤的保护作用

Protective effects of extract from Tribulus terrestris L on adriamycin induced damage of myocardial mitochondria in rats

目的:研究蒺藜提取物对阿霉素(ADR)所致大鼠心肌线粒体损伤的保护作用。方法:将蒺藜提取物灌胃于ADR所致心肌线粒体损伤的大鼠,采用电镜对大鼠心肌细胞进行超微结构观察;以Rhodamine 123为荧光探针,用荧光分光光度仪测定心肌线粒体悬液的荧光强度反应线粒体膜电位;采用比色法测定心肌线粒体中谷胱甘肽过氧化物酶(GSH-Px)活性。结果:ADR累积用量20mg/kg可致大鼠心肌线粒体损伤,膜电位下降,GSH-Px活性降低。蒺藜提取物中、高剂量组(30mg/kg、45mg/kg)可减轻心肌细胞超微结构的损伤,升高心肌线粒体的膜电位和GSH-Px活性。结论:蒺藜提取物能明显减轻ADR所致大鼠心肌线粒体的损伤,对ADR心脏毒性具有保护作用。

AIM ： To study the protective effects of extract from Tribulus terrestris L on adriamycin-induced damage to myocardial mitochondria in rats. METHODS： Rats were given intraperitoneal injection of adriamycin （ADR） to induce the damage to myocardial mitochondria. After continuous ig of extract from Tribulus terrestris L, the every experimental group was observed myocardial ultrastructure with electron microscope, the mitochondrial membrane potential （MMP） and the activity of glutathione peroxidase （GSH-Px） ill myocardial mitochondria were determined. RESULTS： ADR of 20 mg/kg was given to rats intermittently induced severe damage to myocardial mitochondria in rats, decreased MMP and the activity of GSH-Px. Extract from Tribulus terrestris L middle- and high-dosage groups （30 mg/kg, 45 mg/kg） could reduce cardiotoxicity of ADR at the uhrastructure level, and in- crease MMP and the activity of GSH-Px. CONCLUSION： Extract from Tribulus terrestris L may decreased the damage to myocardial mitochondria induced by adriamycin, and has a protective effect on adriamycin-induced cardiotoxicity.