一种快速测定心通口服液中葛根素含量的新方法

Method for rapid analysis of puerarin in Xintong Oral Liquid by AOTF-near infrared spectroscopy

目的:应用声光可调滤光器-近红外光谱技术和化学计量学方法建立一种快速测定心通口服液中葛根素含量的新方法。方法:依据2005版《中国药典》心通口服液含量测定方法,以HPLC测定其葛根素的含量,运用偏最小二乘法建立葛根素近红外光谱与HPLC分析值之间的数学模型,对未知样品进行含量预测。结果:建立的定量模型准确性好,葛根素的内部交叉验证均方差是RMSEP=0.1371,决定系数R2=0.9845,经对外部验正集样品进行外部验证,葛根素的近红外光谱预测值与HPLC分析值之间相关性良好,相关系数是r2=0.9964,方法精密度RSD为1.98%(n=20),方法稳定性RSD为1.57%(n=5)。结论:该方法是一种简便,快速,低耗的新型分析技术,可用于心通口服液的快速定量分析。

AIM ： To establish a new method of rapid analysis of puerarin in Xintong Oral Liquid by acousto-op- tic tunable fiher-nearinfrared spectroscopy. METHODS： HPLC was used as a reference method to determine puer- arin content in Xintong Oral Liquid. Calibration model based on partial least squares（ PLS1 ） algorithm was devel- oped to correlate the spectra and the corresponding values determined by the reference methods. RESULTS： The root mean square error of prediction（RMSEP） of the model for puerarin was 0. 137 1. The determination coefficient was R2 = 0.984 5. The correlation coefficient of the true value and predication value from validation was r2 = 0.996 4. CONCLUSION： The method is a quick, simple and low cost assay and able to be used in quantitative analysis of Xintong Oral Liquid.