氯化两面针碱的体内抗肿瘤作用及其机制

Antitumor effect and mechanism of nitidine chloride

目的研究氯化两面针碱的体内抗肿瘤作用及其对肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)的影响,探讨其作用机制。方法以S180荷瘤小鼠为研究对象,给予不同剂量的氯化两面针碱10d,称取瘤重,计算抑瘤率,同时检测血清TNF-α、IL-2的含量。结果2.5、5.0、10mg·kg-1氯化两面针碱对S180肉瘤生长的抑制率分别为1.95%、27.3%、42.9%,氯化两面针碱组荷瘤小鼠血清中TNF-α、IL-2的含量明显高于生理盐水组。结论两面针碱具有抗肿瘤的作用,TNF-α、IL-2的释放可能是其抗肿瘤作用机制之一。

OBJECTIVE To investigate the antitumor effect of nitidine chloride and its influence on TNF-α,IL - 2 and discuss its potential mechanism. METHODS The mice bearing sarcoma 180 were administrated with different doses of nitidine chloride for 10 days. Then the weight of tumor were measured. The levels of TNF -α and IL - 2 in serum were also determined by enzyme linked immunosorbent assay（ELISA） . RESULTS Nitidine chloride significantly inhibited the growth of S180 bearing in mice The inhibitory rate at the dose of 2.5,5,10 mg · kg-1 was 1.95% ,27.3% ,42.9%, respectively. The levels of TNF -α and IL- 2 were higher obviously in the nitidine chloride group than that in the control group. CONCLUSIONS Nitidine chloride has anti - tumor effect, and the release of TNF -α and IL - 2 may be one of its antitumor mechanism.