RP-HPLC法检测华中五味子药材中的五味子醇甲的含量

Determination of schisandrin in Schisandra sphenanthera by RP-HPLC

研究了RP-HPLC法检测五味子药材中的五味子醇甲含量的方法．色谱条件为色谱柱VP-ODS柱（150mm×4．6mm，5μm）；流动相：V（甲醇）：V（水）=13：7；检测波长250nm；流速1．0mL／min．检测结果为峰面积与浓度线性回归方程Y=2×10^6X＋30294（r=0．9995，n=7），五味子醇甲在0．03～3．0mg／mL范围内线性良好，加样回收率101．01％，RSD为1．829％．研究结果表明，本方法是测定五味子药材中的五味子醇甲含量的理想方法，但不适合测定药材中的五味子乙素含量．

The RP-HPLC method of determining schisandrin in Schisandra sphenanthera is studied by using VP-ODS Column （150 mm × 4. 6 mm, 5 μm） and methanol-water（13 ： 7）as a mobile phase under the detection wavelength at 250 nm and flowing rate of 1.0 ml/min. The linearity regression equation between the concentration of schisandrin and peak area is Y= 2 × 10^6 X＋ 30 294 （r= 0. 999 5, n=7）. The linear correlation is available between 0.03 and 3. 0 mg/mL of schisandrin. The average recovery of schisandrin is 101.01%, and RSD is 1. 829%. The results show that HPLC is a good method for the determination of schisandrin in Schisandra sphenanthera, but not suitable for kadsuranin.