非小细胞肺癌患者外周血细胞中p53基因突变的研究

The p53 gene mutation in peripheral blood cells of non-small cell lung cancer patients and its clinical significance

目的探讨非小细胞肺癌(NSCLC)患者外周血细胞中p53基因突变检测的临床意义。方法收集32例NSCLC患者和30例健康者的外周血标本,采用PCR结合DNA测序方法检测血标本中p53基因突变,然后进行统计学分析。结果①肺癌组32例患者血标本共有12例检测到p53基因突变,突变率为37.5%;健康组30例血标本没有检出p53基因突变。②肺癌患者血液p53基因突变率在(Ⅰ+Ⅱ)期为9.1%、(Ⅲ+Ⅳ)期为52.4%,差异有统计学意义(P<0.05)。结论检测外周血中p53基因突变对于肺癌的诊断、发现远处转移和估计预后具有重要的意义。

Objective To study the clinical significance of detecting p53 gene mutation in peripheral blood of non-small cell lung cancer（NSCLC） patients. Methods Peripheral blood samples of 32 NSCLC patients and 30 healthy volunteers were collected, p53 gene mutation in blood was detected by polymerase chain reaction （PCR）and DNA sequencing method. Results （1） The p53 gene mutations were detected in blood from 12 of 32 NSCLC patients, it＇s mutation rate was 37.. 5%. The p53 gene mutations were found in blood of none of the 30 healthy volunteers. （2） The mutation rates of patients with stages（ Ⅰ ＋ Ⅱ ） and（ Ⅲ ＋Ⅳ） were 9. 1% and 52.4% （P 〈 0. 05 ）. Conclusion Detection of p53 gene mutation in peripheral blood is helpful to diagnosis of lung cancer, estimation of distal metastasis of cancer ceils and evaluating prognosis.