肺癌患者血浆长链DNA的检测及分析

Detection of plasma DNA integrity in lung cancer patients

目的探讨长链DNA作为肿瘤标志物的可能性。方法用实时PCR检测45例肺癌患者(肺癌组)和45例非肿瘤患者(对照组)血液中长链DNA的浓度及其指数。结果肺癌组平均长链DNA指数明显高于对照组,差异具有显著性(P<0.01),在ROC曲线上,假设长链DNA对肺癌特异性是100%,则其临界点为0.59,用于肺癌诊断的敏感性为67%。结论血浆长链DNA也许可作为肺癌诊断的辅助标志物。

Objective To test the hypothesis that increased DNA integrity, ie, a longer DNA stand be a tumor - associated marker in plasma DNA. Methods Using real-time PCR assay, a DNA integrity index and DNA concentration in plasma were determined in 45 patients with lung cancer and 45 patients without neoplastic disease. Results The median DNA integrity index in the neoplastic group was significantly higher than that in the noneoplastlc group （P 〈0. 01 ）. Under the receiver-operating characteristic curve, given that specificity was 100%, the highest sensitivity achieved in detecting the cancer group was 67% （95% confidence interval =0. 541 -0. 74） at the index cutoff of 0. 59. Conclusion Our findings suggest that increased DNA integrity in plasma is associated with cancer, and detection of DNA integrity may provide a simple and inexpensive method for cancer diagnosis.