外用原花青素对皮肤急性光损伤日晒伤细胞及p53蛋白表达的影响

Influence of grape seed proanthoeyanidin extract on sunburn cell formation and p53 protein expression induced by acute ultraviolet injury

目的评估外用葡萄籽提取物原花青素对急性光损伤的日晒伤细胞及p53蛋白表达的影响。方法实验设计正常皮肤组、单纯2MED照射组、基质＋2MED照射组、含葡萄籽提取物原花青素样品外用＋2MED照射组，连续3天相同方法处理后24h对皮肤取材，进行HE染色和p53免疫组化染色。结果含葡萄籽提取物原花青素样品外用＋2MED照射组的日晒伤细胞数与单纯接受紫外线照射的皮肤相比，差异有统计学意义（P〈0.01）。含葡萄籽提取物原花青素样品外用＋2MED照射组的p53蛋白阳性细胞数与单纯接受紫外线照射的皮肤相比，差异有统计学意义（P〈0．05）。结论葡萄籽提取物原花青素外用对紫外线造成的急性光损伤具有较好的防护作用，可作为一种天然的防晒成分加以开发。

Objeetive To evaluate the influence of grape seed proanthocyanidin extract （GSPE） on sunburn cell formation and p53 protein expression induced by acute ultraviolet injury. Methods Ten volunteers were enrolled in this study. The buttock region served as the exposed region. Four areas were randomized and delineated on the buttock： one area （control area） received no exposure or product, the other 3 areas were exposed to two minimal erythema doses （MED） of simulated solar radiation （SSR） for 3 days. Of the 3 exposed areas, one area （SSR） received no product before exposure, one area （SSR ＋ Veh） was pretreated with vehicle, the third area （SSR ＋ GSPE） with the samples of GSPE. GSPE or vehicle was applied 30 minutes before each exposure at 2 ixL/cm2. Skin biopsy was performed 24 hours after the last exposure, and skin specimens were subjected to hematoxylin eosin （HE） staining and histochemical analysis for p53 protein. Results There was a statistical difference in the number of sunburn cells per high power field （×200） between SSR sites and SSR ＋ GSPE sites （29.8 ± 1.1 cells vs 2.2 ±0.2 cells, P 〈 0.01 ）. A significant decrease was noticed in the account of p53 protein-positive cells per high power field （×200） in SSR ＋ GSPE sites compared with the SSR sites （4.6 ± 0.7 cells vs 19.3 ± 3.4 cells, P 〈 0.05）. Conclusion GSPE exerts a potential protective effect against acute ultraviolet injury and can serve as a natural sunscreen.