Gravin在瘢痕疙瘩中的表达

mRNA and protein expression of gravin in keloid

目的探讨Gravin在瘢痕疙瘩形成中的可能作用机制。方法荧光定量PCR法检测并比较Gravin在瘢痕疙瘩和正常皮肤巾的表达情况，免疫荧光双标法分析Gravin在正常皮肤和瘢痕疙瘩中的定位。结果荧光定量PCR结果显示：Gravin在瘢痕疙瘩中的表达量明显降低（0．0953±0．0664），与正常皮肤相比（0.4565±0．1728）差异有统计学差异（P〈0．01）。免疫荧光双标结果显示：正常皮肤组织中，Gravin主要定位于成纤维细胞；在瘢痕疙瘩中，Gravin定位于巨噬细胞和成纤维细胞，主要位于巨噬细胞。结论瘢痕疙瘩中Gravin表达量明显下降且主要定位于巨噬细胞。这种表达和定位的改变可能通过影响瘢痕疙瘩中成纤维细胞和炎症细胞的增殖及活化，参与瘢痕疙瘩的形成。

Objective To study the expression of gravin in the keloid and to investigate its probable role in the pathogenesis of keloid. Methods Skin biopsy was performed and skin samples were obtained from 18 normal human controls and 25 patients with keloid. The mRNA expression of gravin in specimens was detected by real-time fluorescent RT-PCR, and double immunofluorescence analysis was used to investigate the distribution of gravin protein in skin tissues. Results As detected by real time PCR, the relative expression of gravin mRNA was remarkably reduced in comparison with normal skin （0.4565± 0.1728 vs 0.0953 ± 0.0664, P 〈 0.01 ）. Results of double staining indicated that, in normal skin, gravin was primarily distributed in the cytoplasm of fibroblasts; while in keloid, it was observed mainly in macrophages and sporadically in fibroblasts. Conclusions Compared with the normal skin, keloid shows a different expression intensity and distribution of gravin, which might contribute to the development of keloid by regulating the proliferation of fibroblasts and activation of macrophages.