罗格列酮钠对非酒精性脂肪肝大鼠COX-2表达的影响

Effect of Rosiglitazone on the expression of COX-2 protein in non alcoholic fatty liver disease rats

目的探讨罗格列酮钠(RSG)对高脂诱导的非酒精性脂肪肝(NAFLD)大鼠COX-2表达的影响。方法30只SD大鼠分为对照(CON)组、高脂(HFD)组、RSG组。高脂饮食8w建立大鼠NAFLD模型,建模后,RSG组灌胃3mg·kg-1.d-1RSG,CON组及高脂组灌等量蒸馏水,共12w。测定丙二醛(MDA)、超氧化物歧化酶(SOD)、C反应蛋白(CRP)。光镜观察形态学变化,免疫组化法观察COX-2的表达。结果与CON组相比,HFD组的MDA、CRP升高(P<0.01),SOD降低(P<0.01),HFD组出现明显的脂肪变性(P<0.01),COX-2表达明显增高(P<0.01)。与HFD组相比,RSG组的MDA、CRP降低(P<0.01),SOD升高(P<0.01),肝脏脂肪变性减轻(P<0.01),COX-2表达下降(P<0.01)。结论RSG可以减轻高脂诱导的NAFLD大鼠COX-2的表达,从而减轻NAFLD的炎症。

Objective To explore the effect of Rosiglitazone （RSG） on the expression of COX-2 protein of non alcoholic fatty liver disease （NAFLD） rats induced by high fat diet. Methods Thirty SD rats were divided into control, high fat diet and RSG groups. After the animal model of NAFLD） was induced by fed with high fat diet for 8 weeks, RSG group was treated by garage with RSG for 12 weeks. MDA, SOD and CRP were examined, morphological changes were observed by light microscope, the expression of COX-2 protein was measured by irnmunohistochemistry. Results （1）The levels of MDA and CRP of high fatty diet group were significantly higher and the level of SOD was lower than those of control group （P 〈 0. 01 ）. Typical steatosis was aggravated and the expression of COX-2 was increased in high fatty diet group than those in control group. （2） The levels of MDA and CRP of RSG group were significantly lower and the level of SOD was higher than those of high fatty diet group （P 〈 0. 01 ）. The degree of liver steatosis was significantly alleviated and the expression of COX-2 was decreased in RSG group than those in high fatty diet group （P 〈0. 01 ）. Conclusions RSG could attenuate inflammation reaction of NAFLD by alleviating the expression of COX-2.