摘要
目的探讨血红素氧合酶-1(HO-1)基因启动子(GT)n多态性对冠心病患者冠脉粥样硬化病变程度及病变稳定性的影响。方法通过冠脉造影选择冠心病患者126例,采用Gensini评分系统对冠脉病变进行评分,提取外周静脉血DNA,PCR法扩增含HO-1基因启动子(GT)n的片段,采用测序加聚丙烯酰胺凝胶电泳法确定HO-1基因启动子(GT)n,同时检测血hs-CRP、IL-6、MMP9的浓度。结果短(GT)n携带者较非短(GT)n携带者冠脉病变评分、ACS发生比例及血hs-CRP、IL-6、MMP6浓度均明显降低。结论HO-1基因启动子(GT)n多态性对冠脉粥样硬化病变有明显影响,短(GT)n基因型能减轻冠脉粥样硬化病变程度,并可能通过抗炎机制保持病变稳定。
Objective To explore the effects of heme oxygenase-1 （ HO-1 ） gene promoter （GT） n polymorphism on the severity and stability of coronary atherosclerosis in patients with coronary heart disease （CHD）. Methods 126 patients with CHD by coronary angiography were selected. The severity of coronary artery disease （CAD） was defined by Gensini score system. DNA was abstracted from peripheral venous blood and HO-1 gene promoter （GT）n fragment was augmented by PCR. Sequencing added polyacrylamide gel electrophoresis was used to define HO-1 gene promoter （GT）n. The content of highly sensitive C-reactive protein （hs-CRP）, interleukin-6 （IL-6） and matrix metalloproteinase-9 （MMP-9） were also detected. Results Compared with non-short （GT）n genotype carriers, the carriers with short （GT） n genotype had significantly lower Gensini score, less proportion of acute coronary syndrome and lower concentration of hs-CRP, IL-6 and MMP-9 （all P 〈 0. 05 ）. Conclusions HO-1 gene promoter （GT）n polymorphism has obvious effect on CAD. Short （GT）n genotype can alleviate the atherosclerosis severity and increase the stability of CAD by anti-inflammatory.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第3期315-317,共3页
Chinese Journal of Gerontology
基金
四川省卫生厅科学研究基金项目(030078)
四川省教育厅自然科学科研项目(2003B007)
