乳腺浸润性导管癌微血管生成与转录因子Ets1表达之间的关系

Relationship between Tumor Angiogenesis and Transcription Factor Ets1 in Breast Infiltrating Ductal Carcinoma

目的:探讨乳腺导管上皮癌变过程中微血管的分布特点及转录因子Ets1和其下游蛋白MMP1对肿瘤血管生成的作用。方法:应用免疫组织化学SP法检测正常乳腺18例(NB组)、导管上皮普通性增生20例(UDH组)、非典型增生29例(ADH组)、导管原位癌15例(DCIS组)和浸润性导管癌70例(IDC组)中CD34标记的微血管密度(MVD)、转录因子Ets1和其下游蛋白MMP1的表达,并应用CMIAS计算机辅助病理图像分析系统对结果进行分析。结果:CD34标记的MVD随着导管上皮增生病变程度的加重依次增加,差别具有统计学意义(均P<0.01);转录因子Ets1和其下游蛋白MMP1在IDC组中的表达明显高于导管内增生性病变(均P<0.01),且两者的表达呈正相关(r=0.55,P<0.01);IDC组中CD34标记的MVD与Ets1和MMP1均有相关性(r分别为0.57和0.60,均P<0.01)。结论:Ets1可能是调控乳腺癌血管生成的关键点。

Objective： To detect the expression of microvessel density （MVD） and transcription factor Ets1 in breast intraductal proliferative lesion and infihrating ductal carcinoma. Methods： Immunohistochemistry and computer-assisted image analysis system were used to detect the expression of MVD and transcription factor Ets1 and the downstream protein MMP1 in 152 routinely paraffin embedded specimens which included 18 cases of normal breast （NB group） ,20 cases of usual ductal hyperplasia （UDH group）, 29 cases of atypical ductal hyperplasia （ADH group）, 15 cases of ductal carcinoma in situ （DCIS group） ,and 70 cases of infiltrating ductal carcinoma （IDC group）. Results： MVD showed an increasing trend from normal breast to infiltrating duetal carcinoma, and a significant increase was showed in IDC group. The positive expression of Ets 1 and MMP1 was increased according to the degree of the hyperplasia of breast intraductal epithelium, and in IDC group exhibited a significantly increased immunoreactivity compared with intraductal proliferative lesion （P 〈 0.01）. In IDC group, Ets1 significantly correlated with MMP1 （r = 0.55, P 〈 0.01）. Etsl and MMP1 were all significantly correlated with MVD marked by CD34 （r = 0.57, r = 0.60, and respectively, P 〈 0.01）. Conclusion： Etsl may play an important role in regulating angiogenesis in breast cancer.