摘要
目的:探讨芍药甘草汤(芍甘汤)的抗炎作用及其机制。方法:采用角叉菜胶诱发大鼠足肿胀和大鼠背部气囊模型、巴豆油混合致炎剂诱发小鼠耳肿胀模型以及鲁米诺化学发光法测定多形核白细胞(PMN)的发光强度。大鼠气囊炎性渗出液中前列腺素E2(prostaglandin E2,PGE2)、白细胞介素-6(interleukin,IL-6)和大鼠血清皮质醇用放射免疫法测定,一氧化氮(nitric oxide,NO)用硝酸还原酶法测定。结果:芍甘汤对大鼠足肿胀、小鼠耳肿胀有显著抑制作用,能明显减少气囊炎性渗液中的PGE2、IL-6、NO,降低气囊模型大鼠血清皮质醇含量,芍甘汤药物血清和芍甘汤有效成分芍药苷能明显抑制激活的PMN化学发光。结论:芍甘汤有明显的抗炎作用,其抗炎作用机制部分在于抑制PGE2、NO、IL-6的产生,抑制PMN产生氧自由基,可能还与影响下丘脑-垂体-肾上腺皮质轴有关。
Objective： To explore the mechanism of Shaogan decoction effects on anti-inflammation. Methods： Rat footswelling model and rat back air-cyst model induced by carrageenin and mouse ear-swelling model induced by croton oil mixed inflammatory agent were used. Polymorphonuclear neutrophil leukocyte （neutrophil, PMN） luminous intensity was determined tbrougb luminal chemiluminescence. Prostaglandin E2 （PGE2） and interleukin-6 （IL-6） in rat air-cyst inflammatory effusion and rat serum cortisol were determined with radioimmunoassay. Nitric oxide （NO） was determined by nitrate reduetase method. Results： Shaogan decoction inhibited remarkably rat foot-swelling and mouse ear swelling, decreased in PGE2, IL-6 and NO in inflammatory effusion, lowered serum cortisol level in air-cyst model rats. Serum containing drugs from rats and effective composition peoniflorin of shaogan decoction inhibited markedly luminous intensity of activated PMNs. Conclusion： Shaogan decoction has a remarkable anti-inflammation effect. Its anti-inflammation mechanism is related to inhibiting inflammatory media （PGE2）, cytokine （IL-6） and NO production, inhibiting oxyradical production by PMN, lessening stress reaction to inflammation by interfering hypothalamo-pituitary-adrencortical.
出处
《天津医药》
CAS
北大核心
2009年第2期120-123,共4页
Tianjin Medical Journal
基金
天津市科技攻关计划重大科技项目(项目编号:05YFGDSF02600):分项目按治则归类进行新药开发与作用机制研究
