摘要
目的探讨苏拉明辅助用于兔小梁切除术中的抗增殖效果。设计实验研究。研究对象新西兰白兔32只。方法32只兔双眼均行标准小梁切除术,并随机分为空白对照组、标准对照组、实验Ⅰ组、实验Ⅱ组,后3组术中巩膜瓣下分别应用0.3mg/ml丝裂霉素C、220 mg/ml及250 mg/ml苏拉明海绵片2 min。术后第3、7、15、30d观察眼压及滤过泡形态并行HE染色和增殖细胞核抗原(PCNA)染色。主要指标眼压、滤过泡形态、组织病理染色。结果术后30天空白组和实验Ⅰ组功能滤过泡比率均较标准组低,实验Ⅱ组高于其它三组。PCNA染色阳性细胞核计数(四组分别为18.79±1.91,11.75±1.96,11.08±1.80,5.12±1.15)实验Ⅰ组和标准组无统计学差异(P>0.05),两组均较空白组少,实验Ⅱ组最少(P<0.05)。结论苏拉明辅助用于兔小梁切除术可维持功能性滤过泡,250 mg/ml苏拉明抗增殖能力不低于0.3mg/ml丝裂霉素C,而副作用小。
Objective To investigate the antiproliferative effect of suramin on trabeculectomy in rabit. Design Experimental study. Participants 32 New Zealand albino rabbits. Methods 32 albino rabbits were randomly divided into four groups. Both eyes of rabbits were performed trabaculectomy and placed a piece of cotton with 0.3 mg/ml mitomycin C （MMC） for 2 minutes in standard control group, 220 mg/ml suramin for 2 minutes in experimental group Ⅰ , 250 mg/ml suramin for 2 minutes in experimental group Ⅱ. The examination of intraocular pressure was performed before surgery. The filtration bleb and intraocular pressure were observed at 3, 7, 15, 30 days after surgery. Some conjunctiral specimen was observed with histocbemicall （HE staining） and immunohistochemicall （PCNA staining） methods. Main Outcome Measures Intraocular pressure （IOP）, filtering bleb appearance, histopathological staining. Results The percentage of the functional blebs in empty control group and experimental group I was lower than in standard control group, and experimental group Ⅱ was higher than in the other 3 groups at 30 days after surgery. Immunohistochemical staining showed the number difference of PCNA positive cell nuclear was no significant between experiment group Ⅰ and standard control group, but less than empty control group, and experiment group Ⅱ was the least （P〈0.05）. Conclusion Suramin can maintain effectively functional blebs after trabeculectomy in rabit. The efficacy of 250 mg/ml suramin is no lower than that of 0.3 mg/ml MMC.
出处
《眼科》
CAS
2009年第1期51-54,共4页
Ophthalmology in China
