淮南猪瘦肉型品系应激敏感性的研究

Study on PSS in high lean strain of huainan pig

猪氟烷(halothane,HAL)基因n是猪产生猪应激综合症(PSS)的主效基因,采用限制性核酸内切酶HhaI对淮南猪瘦肉型品系共155头的HAL基因进行PCR-RFLP分析,结果表明:淮南猪新品系HAL基因HhaI酶切的优势基因型为显性纯合子NN,频率为0.9739。淮南猪新品系HAL基因HhaI酶切没有产生隐性纯合子基因型nn,新品系猪杂合子基因型Nn和显性纯合子NN不产生应激综合症(PSS)。新品系保持了淮南猪无应激敏感这一优良特性。

Porcine stress syndrome（PSS） is induced by major gene -halothane（HAL） gene. The polymorphisms of HAL gene were analyzed by PCR-RFLP wtih I-Ihal in 155pigs from high lean strain of Huainan pig. The result showed that： Dominant genotype in quantity is NN at 95% degree in high lean strain of Huainan pig;nn genotype was zero, PSS wasnot detected in high lean strain of Huainan pig with Nn genotype and all NN genotype, It showed the new strain retained superior trait of Huainan pig.