食管鳞状细胞癌组织中KISS-1和MMP-9蛋白和mRNA的表达

Expressions of KISS-1 and MMP-9 in esophageal squamous cell carcinoma tissue

目的:探讨肿瘤转移抑制基因KISS-1及基质金属蛋白酶-9(MMP-9)与食管鳞状细胞癌侵袭、转移的关系。方法:采用免疫组织化学SP法和逆转录聚合酶链反应(RT-PCR)检测50例食管鳞状细胞癌及50例正常食管组织中KISS-1及MMP-9蛋白和mRNA的表达情况,分析其与食管鳞状细胞癌患者各临床病理特征的关系及二者的相关性。结果:食管鳞状细胞癌组织中KISS-1蛋白的阳性表达率(61.0%)低于正常食管组织(82.0%)(χ2=5.877,P<0.05),并且其低表达与淋巴结转移密切相关(χ2=4.340,P<0.05)。食管鳞状细胞癌组织中MMP-9蛋白的阳性表达率(72.0%)高于正常食管组织(52.0%)(χ2=4.244,P<0.05),并且其高表达与肿瘤的浸润、淋巴结转移及分化程度均相关(P均<0.05)。食管鳞状细胞癌组织中KISS-1mRNA的阳性表达率(56.0%)及表达水平(0.992±0.090)均低于正常食管组织(88.0%,1.075±0.181)(P<0.05),并且其低表达也与淋巴结转移密切相关(P<0.05)。食管鳞状细胞癌组织中MMP-9mRNA的阳性表达率(72.0%)及表达水平(1.030±0.153)均高于正常食管组织(44.0%,0.943±0.085)(P<0.05),并且其高表达与肿瘤的深层浸润和淋巴结转移密切相关(P<0.05)。KISS-1与MMP-9蛋白和mRNA的表达均呈负相关(P<0.05)。结论:KISS-1的低表达和MMP-9的过表达可能与食管鳞状细胞癌的浸润、转移有关。二者有望成为判定食管鳞状细胞癌侵袭和转移能力的指标。

Aim： To explore the relationship between metastasis suppressor gene KISS-1, matrix metalloproteinase-9 （MMP-9） and the invasion, metastasis of esophageal squamous cell carcinoma. MethOdS： The expressions of KISS-1 and MMP-9 protein and mRNA in specimens taken from 50 cases of esophageal carcinoma and 50 cases of normal esophageal mucosa were detected by immunohistochemieal assay （SP） and reverse transcription-polymerase chain reaction （RT-PCR）. To analyze the relationship between them and clinical data. Results： The positive expression rate of KISS-1 protein in esophageal squamous cell carcinoma （61.0%） was significantly lower than that in normal esophageal mucosa （82.0%） （ P 〈 0.05 ）. The low expression of KISS-1 protein in esophageal squamous cell carcinoma was related with lymph node metastasis （P 〈 0.05 ）. The positive expression rate of MMP-9 protein in esophageal squamous cell carcinoma （72.0%） was significantly higher than that in normal esophageal mucosa （52.0%） （ P 〈 0.05）. The high expression of MMP-9 protein in esophageal squamous cell carcinoma was related with differentiation and the depth of invasion and the metastasis of lymph node （ P 〈 0.05 ）. The positive expression rate and relative contents of KISS-1 mRNA in esophageal squamous cell carcinoma （56.0% , 0. 992 ± 0. 090） were significantly lower than those in normal esophageal mueosa （88.0% , 1. 075 ± 0.181 ） （ P 〈 0.05 ）. The low expression of KISS-1 mRNA in esophageal squamous cell carcinoma was related with lymph node metastasis （ P 〈 0.05 ）. The positive expression rate and relative contents of MMP-9 mRNA in esophageal squamous cell carcinoma （72.0% , 1. 030 ± 0. 153 ） were significantly higher than those in normal esophageal mucosa （44.0% , 0. 943 ± 0. 085） （ P 〈 0.05）. The high expression of MMP-9 mRNA in esophageal squamous cell carcinoma was related with the depth of invasion and the metastasis of lymph node（ P 〈 0.05）. The expression of KISS-1 had negative correlation with the expression of MMP-9 （ P 〈 0.05 ）. Conclusion ： Low expression of KISS-1 and high expression of MMP-9 may be related with invasion and metastasis of esophageal carcinoma. KISS-1 and MMP-9 could be a marker to evaluate invasion and metastasis in esophageal squamous cell carcinoma.