肺鳞癌组织中缝隙连接蛋白43的表达

Expression of Connexin43 in lung squamous cell carcinoma

目的:检测肺鳞癌组织及癌旁正常肺组织中缝隙连接蛋白43(Connexin43)蛋白及mRNA的表达。方法:应用免疫组织化学SP法和原位杂交方法检测52例肺鳞癌组织及28例正常肺组织中Connexin43蛋白及mRNA的表达情况,并分析其表达与肺鳞癌转移的关系。结果:①正常肺组织和肺鳞癌组织中Connexin43蛋白阳性表达率分别为93%(26/28)和48%(25/52);mRNA的阳性表达率分别为96%(27/28)和54%(28/52)。肺鳞癌组织中Connexin43蛋白及mRNA阳性表达率均低于正常肺组织(P均<0.05)。②有淋巴结转移组肺鳞癌组织中Connex-in43蛋白及mRNA阳性表达率均为28%(7/25);无淋巴结转移组Connexin43蛋白及mRNA阳性表达率均为35%(16/27)和67%(18/27)。2组比较差异均有统计学意义(P均<0.05)。结论:Connexin43蛋白和mRNA低表达与肺鳞癌发生、发展及浸润、转移有关。

Aim ： To detect the expression of Connexin43 protein and mRNA in lung squamous cell carcinoma. Methods：The expressions of Connexin43 protein and mRNA in 52 cases of lung squamous cell carcinoma,28 cases of normal tissue were detected by using immunohistoehemistry and in situ hybridization. The relationship between the expression of Connexin43 protein or mRNA and angiogenesis,progression and metastasis of lung squamous cell carcinoma. Results ：The positive expression rate of Connexin43 protein and mRNA were 92.86% （26/28）and 96.43% （27/28）in normal tissue. The positive expression rate of Connexin43 protein and mRNA were 48.08% （25/52）and 53.85% （28/52）in lung squamons cell carcinoma tissue. The positive rate of Connexin43 protein and mRNA in lung squamous cell carcinoma tissue was significantly lower than those in normal tissue （P 〈 0.05 ）. The positive rates of Connexin43 protein and mRNA in metastatic group were lower than those in non-metastatic group （ P 〈 0.05 ）. Conclusion ： The expression of Connexin43 protein and mRNA in human lung squamous cell carcinoma tissue is decreased. The expression of Connexin43 protein and mRNA is related to development, invasion and metastasis of human lung squamous cell carcinoma.