胃癌组织中层黏连蛋白受体67LR和半乳糖凝集素-3蛋白的表达

Expression of 67LR and Galectin-3 in the patients with gastric carcinoma

目的:了解胃癌组织中67000层黏连蛋白受体(67LR)和半乳糖凝集素-3(Galectin-3)蛋白的表达情况。方法:用免疫组织化学SP法检测82例胃癌组织和20例胃癌手术远端切缘正常胃组织中67LR蛋白和Galectin-3蛋白的表达,并分析其与胃癌患者临床病理特征的关系。结果:胃癌组织中67LR蛋白的阳性表达率为59.8%,Galectin-3蛋白的阳性表达率为84.1%,均高于相应的正常胃黏膜组织中的阳性表达率(30.0%和35.0%)。胃癌组织中67LR与Galectin-3蛋白的阳性表达率与患者的年龄、性别、Lauren分型无关;67LR蛋白的阳性表达率与胃癌的组织分化程度有关(P<0.05),Galectin-3蛋白的阳性表达率与胃癌的组织分化程度无关;有淋巴结转移的癌组织中两者的阳性表达率均高于无淋巴结转移组(P<0.05);随着肿瘤浸润深度的增加,两者的阳性表达率均明显增高(P<0.05)。胃癌组织中67LR与Galectin-3的表达呈正相关(P<0.05)。结论:67LR及Galectin-3蛋白阳性表达率与胃癌的侵袭、转移密切相关。

Aim ： To study the expression of 67LR, galectin-3, and their relationships in patients with gastric carcinoma. Methods：The expression of 67LR and galectin-3 by immunohistochemical staining with the correspoding monoclonal antibodies, and their relation with the clinicopathologic characteristics were analyzed in 82 patients of primary gastric carcinoma. Gastric tissues were randomly collected from 20 patients as the controls which were gastric mucosas from the distant cutting margin. Results： The positive rate of expression of 67LR and galectin-3 were 59.8% （49/82） and 84.1% （69/82） in gastric cancer cases, respectively, whereas the epithelial ceils of gastric mucosas from the distant cutting margin had weak immunoreactivity which were 30.0% and 35.0% , respectively. The expression of 67LR and galectin-3 in gastric carcinogen tissues were not related to gender, age, and Lauren classification. The expression phenotype of 67LR but not Galectin-3 was correlated with cell differentiation. Moreover, the expression levels of 67LR and galectin-3 were stronger in gastric cancerous tissues with lymph node metastasis than that in the tissues with no lymph node metas＇tasis（ P 〈 0.05 ）. Both of the 67LR and galectin-3 expression levels were significantly increased with the depth of tumor invasion （ P 〈 0.05 ）. Conclusion ： The expression of 67LR and galectin-3 might be closely associated with the invasion and metastasis of gastric cancer.