宫颈癌组织中DNA甲基转移酶1mRNA的表达

Expression of DNMT1 mRNA in uterine cervical carcinoma tissue

目的:探讨DNA甲基转移酶1(DNMT1)在宫颈癌发生发展中的作用。方法:应用原位杂交技术检测52例宫颈癌、60例宫颈上皮内瘤变和20例正常宫颈组织DNMT1 mRNA的表达;分析DNMT1 mRNA的表达和宫颈癌患者临床病理指标的关系。结果:正常宫颈、宫颈上皮内瘤变和宫颈癌组织中DNMT1 mRNA的阳性率分别为10.0%(2/20)、63.3%(38/60)和78.8%(41/52),呈升高趋势(χ2=29.057,P<0.05),宫颈上皮内瘤变和宫颈癌组织DNMT1 mRNA的阳性率高于正常组织。不同宫颈癌的病理类型、临床分期、组织学分级及有无淋巴结转移组间DNMT1 mRNA阳性率差异无统计学意义。结论:DNMT1参与了宫颈癌的发生,并在宫颈上皮内瘤变时就已经起了重要作用。

Aim ： To study the relationship between DNMT1 expression and oncogenesis of uterine cervical carcinoma. Methods：The expression of DNMT1 mRNA was detected by in situ hybridization in 52 cases of cervical carcinoma,60 cases of cervical intraepitbelial neoplasia（CIN） and 20 cases of normal cervical tissue. Results： The positive rate of DNMTI mRNA expression in cervical carcinoma and CIN were 78.8% and 63.3% , higher than that（ 10.0% ） in normal cervical tissue （P 〈 0.05 ）. No relationship was observed between DNMT1 mRNA and pathological categories, clinical stages, histological grades or lymph node status（ P 〉 0.05 ）. Conclusion ： Progressively increasing expression of DNMT1 is associated with cervical carcinogenesis even during the precancerous stages.