选择性增菌在产TEM型ESBL菌株痰标本检测中的应用

The application of select cloning in detecting TEM ESBL in sputum

目的:探索选择性增菌在痰标本TEM型ESBL菌株痰标本检测中应用的可行性。方法:从郑州大学第一附属医院30份不重复的痰标本中分离出TEM型ESBLs菌株,采用LB液体培养基进行增菌培养,应用氨苄青霉素(终质量浓度为100mg/L)进行选择抑菌,采用酶抑制剂增强试验及PCR技术进行ESBLs确证。结果:酶抑制剂增强试验中头孢噻肟与头孢噻肟/克拉维酸抑菌环直径差大于5mm;PCR扩增产物在约900bp处有明显条带。结论:选择性增菌方法简单、快速,准确,可用于痰标本中TEM型ESBL菌株的检测。

Aim ：To investigate the application of select cloning in the detection of TEM-type ESBL in sputum. Methods： 30 specimens which contained ESBLs from the First Affiliated Hospital of Zhengzhou University were collected. Cloning was selected by LB culture medium which contains Amp（ 100 mg/L）. Detecting ESBL by enzyme inhibitor enhancing test and PCR. Results ：The diameter size differences between CTX and CD03 exceeded 5 mm. The PCR amplying product was about at 900 bp in agarose electrophoresis. Conclusion ：Select cloning is rapid, accurate and can be used in detecting ESBL in sputum.