柯萨奇B3病毒川金丝猴分离毒株实验性感染BALB/c小鼠的病理形态学

Pathomorphology of BALB/c mice infected experimentally with coxsackievirus B3 from Sichuan golden monkey

目的：研究来源于川金丝猴的柯萨奇B3病毒株CVB／SGM-05对BALB／c小鼠的致病变特点。方法：4-6周龄健康雄性BALB／c小鼠30只，通过腹腔注射途径将滴度为10^5个TCID50／0．1mL的CVB／SGM—05接种小鼠（0．2mL／只），观察感染小鼠的临床症状及大体病变特点；分别摘取心脏、肝脏、脾脏、肺脏、肾脏、胰腺、胃、肠、脑和脂肪组织，经10％的中性甲醛溶液固定后制作常规的病理石蜡切片，光镜下观察其病理变化特点。结果：小鼠于接毒后60h开始死亡，死亡率达80％；发病小鼠均出现典型的结膜炎。对死亡小鼠剖检可见，肝脏明显肿大，呈淡黄色样改变，表面可见针尖大灰白小点；部分小鼠的脾脏肿大、边缘与坏死的脂肪发生粘连；最明显的病变为腹腔内脂肪的大量坏死。病理切片观察可见心、肝、肾组织均有不同程度的细胞变性，心肌纤维间有炎性细胞浸润，肝细胞坏死严重；胃、肠黏膜上皮坏死、脱落；脑组织可见轻度淤血；脂肪坏死尤其明显。结论：与人源的标准毒株Nancy比较，猴源的CVB／SGM-05感染BALB／c小鼠可引起多脏器损伤，提示不同来源的CVB3毒株可能对感染动物的致病性有一定差异。

Objective To study the pathological changes of BALB/c mice infected experimentally with coxsackievirus B3 （CVB/SGM-05） from Sichuan golden monkey. Methods Thirsty male BALB/c mice （4-6 weeks age） were infected with CVB/SGM-05 intraperitoneally （10^5TCID50/0.1 mL, 0.2 mL each mouse）, the clinical symptoms were observed. The pathological changes in heart, liver, spleen, lung, kidney, pancreas, stomach, intestines, brain and fat tissues were fixed in 10% formalin and embedded in paraffin, the sections were deparaffinized and stained with hematoxylin and eosin, and then were examined at different time after infection. Results The different degrees of pathological changes could be definitely observed in various organs of mice with myocarditis and conjunctivitis, and the mice died from the 60th hour after inoculation with CVB/SGM-05, the mortality was 80%.The main lesion was fat necrosis in the abdominal cavity of mice. Liver and spleen were swell, and some small grayish-white loci were observed in the liver. The hepatocyte necrosis was severe. There was light congestion in the brain, the mucous membrane was necrosis and abscission on the stomach and intestine. Oonclusion Compared with the CVB3-Nancy, many tissues and organs are damaged in BALB/e mice infected with CVB/SGM-05, it suggests that CVB3 from the Siehuan golden monkey is different from the other CVB3 in pathogenicity.