羊蹄根提取物对血小板减少症模型小鼠造血系统的作用

Effect of Rumex.japonicus Houtt extracts on hemopoietic system of model mice with thrombocytopenia

目的:研究羊蹄根丙酮、乙醚提取物对小鼠血小板减少症的治疗作用。方法:80只BACB/C小鼠随机分为5组(每组16只):阴性对照组(色拉油)、羊蹄根丙酮提取物中剂量(0.5 g生药.kg-1)、高剂量(5.0 g生药.kg-1)组和乙醚提取物中剂量(0.5 g生药.kg-1)、高剂量(5.0 g生药.kg-1)组。小鼠连续3 d皮下注射环磷酰胺(0.3mL/20g),建立血小板减少症模型;将造模成功小鼠分别给予色拉油,中、高剂量羊蹄根丙酮提取物及中、高剂量羊蹄根乙醚提取物灌胃,20 d后处死动物采血检测血小板数、红细胞数、白细胞数。结果:①与造模前比较,造模后第14天各组小鼠血小板数量降至最低(P<0.01);②造模后第20天(实验结束时)各组小鼠血小板数量高于第14天(P<0.01),且小鼠血小板数羊蹄根丙酮、乙醚提取物各实验组高于阴性对照组(P<0.01);③羊蹄根丙酮、乙醚提取物各实验组小鼠红细胞、白细胞的数量与对照组比较差异无显著性(P>0.05)。结论:中、高剂量羊蹄根丙酮、乙醚提取物对小鼠血小板减少症有明显治疗作用。

Objective To study the treating effect of middle and high doses of acetone and aether extracts from Rumex. Japonicus Houtt on mouse thromboeytopenia. Methods 80 BACB/C mice were randomly divided into 5 groups （n=16）： negative control group （salad oil）, middle dose （0. 5 g· kg^-1） and high dose （5 g · kg^-1） of acetone and aether extracts groups. All mice in various group were injected with cyclophos-phamide （0.3 mL/20 g） by hypodermic for 3 d to set up the thrombocytopenic model; after mouse thrombocytopenic model was successfully set up, the mice were given salad oil, middle and high doses of acetone and aether extracts, respectively, by gastric perfusion, 20 d later the mice were executed, the numbers of the platelet, red cells and white cells were determined. Results ① The numbers of platelet numbers of all groups on the 14th day after making model decreased significantly （P〈 0.01）; ②When the experiment was finished, the platelet numbers of mice were significantly increased compared with the 14th day （P〈 0.01）, the platelet numbers of experimental groups were obviously increased compared with negative control group （P〈0. 01） ③There were no significant differences of the numbers of red cells and white cells between experimental groups and negative control group （P〉0.05）. Conclusion Middle and high doses of acetone and aether extracts have obviously curative effect on mouse thrombocytopenia.