生长抑素受体亚型SSTR2和SSTR5 mRNA在肝癌组织中的表达及其临床意义

Expressions of SSTR2 and SSTR5 mRNA in hepatocellular carcinoma tissues and their chinical significances

目的:分析肝癌组织中生长抑素受体亚型SSTR2和SSTR5 mRNA表达状况及其与肿瘤的关联性,为应用生长抑素及其类似物诊断和治疗肝癌奠定理论基础。方法:肝癌患者肝组织标本22例,采用RT-PCR检测并鉴定肝癌癌组织、癌旁组织及正常肝组织SSTR2和SSTR5 mRNA表达率,分析表达率与肿瘤特征之间的关系。结果:SSTR2 mRNA肝癌癌组织的表达率为81.8%(18/22),表达水平为118.27±17.25,与正常肝组织[36.4%(8/22),69.49±19.43]比较差异有显著性(P<0.05);与癌旁组织[72.7%(16/22),111.18±23.74]比较差异无显著性(P>0.05);癌旁组织与正常肝组织比较差异有显著性(P<0.05)。SSTR5 mRNA表达率和表达水平肝癌癌组织[18.2%(4/22),51.67±30.32]、癌旁组织[18.2%(4/22),49.56±27.61]及正常肝组织[9.1%(2/22),48.73±15.72]三组间两两比较差异均无显著性(P>0.05)。高分化肝癌组织中[SSTR2 100%(18/18),SSTR5 25%(4/16)]和无淋巴结转移的肝癌组织中表达率[SSTR2 25.0%(4/16),SSTR5 100%(4/4)]分别高于低分化的表达率[0(0/4),0(0/6)]和有淋巴结转移的表达率[0(0/6),0(0/18)](P<0.05);Ⅰ、Ⅱ期[SSTR2 85.7%(12/14),SSTR5 21.4%(3/14)]与Ⅲ、Ⅳ期[75.0%(6/8),12.5%(1/8)]表达率比较差异无显著性。结论:SSTR2 mRNA亚型在肝癌中呈高表达,其表达率、表达水平与癌组织分化程度、有无淋巴结转移有关联性,与肝癌的分期无关联。

Objective To detect the expressions of somatostatin receptor subtypes SSTR2 and SSTR5 mRNA in patients with hepatoeellular carcinoma （HCC） and investigate the relationship between expression rates and carcinoma characters, in order to provide theoretical foundation for diagnosis and therapy of HCC. Methods 22 patients with HCC were included, all the specimens with HCC were identified by surgery and the histopathologieal examination. The expressions of SSTR2 and SSTR5 mRNA in carcinoma tissues, adjacent tissues, and normal tissues were determined and identified with RT PCR. The relationship between the expression rate and carcinoma characters was analyzed. Results The expression rate of SSTR2 mRNA in carcinoma tissues was 81.8%, the expression level was 118.27± 17.25, they were obviously higher than those in normal tissues （36.4%, 69.49± 19.43） （P〈0.05）; but there was no difference between carcinona tissues and adjacent tissues （72.7%, 111.18±23.74） （P〉 0.05） ; there was obvious difference between normal tissues and adjacent tissues （P〈0.05） . There was no significant difference of the SSTR5 mRNA expression rates and expression levels between carcinoma tissues （18.2%, 51.67±30.32）, adjacent tissues （18.2%, 49.56±27.61） and normal tissues （9.1%, 48.73±15.72） （P〉 0.05） . The expression rates of SSTR2 mRNA and SSTR5 mRNA in high differentiation HCC tissues （100 % and 25 %） and lymph node metastasis negtive HCC tissues （25.0% and SSTR5 100%） were higher than those in low differentiation HCC tissues （0 and 0） and lymph node metastasis positive HCC tissues （0 and 0） （P〈0.05） ; there was no obvious difference of the expression rates between TNM stage Ⅰ ＋ Ⅱ HCC tissues （SSTR2 85.7%, SSTR5 21.4%） and TNM stage Ⅲ ＋Ⅳ （75.0%, 12.5%） （P〉0.05）. Conclusion The expression level of SSTR2 mRNA in HCC is high. The expression rate and expression level of SSTR2 have close relationship with the differentiantion, lymph node metastsis, and have no correlation with HCC classification.