应用细胞化学法与X-射线能谱仪对深低温停循环后脑神经细胞中Ca^(++)浓度的定量研究

Quantitative Study on The Change of Ca ++ Concentration in Neuron after Deep Hypo thermic Circulatory Arrest by Using The Method of Cytochemistry and Energy Dispersive X ray Microanalysis

用草酸－焦锑酸钾细胞化学法显示深低温（１８℃）停循环（ＤＨＣＡ）１２０ｍｉｎ后脑神经细胞内Ｃａ＋＋的分布及超微结构改变，同时利用Ｘ－射线能谱仪测量细胞内Ｃａ＋＋浓度以及停循环时应用１．６－二磷酸果糖（ＦＤＰ）保护液后细胞内Ｃａ＋＋浓度的变化。结果显示：（ＤＨＣＡ）１２０ｍｉｎ后脑神经细胞超微结构破坏严重，细胞内Ｃａ＋＋浓度明显增加。但在（ＤＨＣＡ）期间应用（ＦＤＰ）脑保护液后可以明显减轻术后神经细胞内Ｃａ＋＋聚积与脑损伤，是（ＤＨＣＡ）下脑保护的可行方法。

The distribution of Ca ++ and ultrastructural changes in brain neuron were determined after 120 minutes of deep hypothermic (18℃) circulatory arrest (DHCA) by using K pyroantimonate cytochemistry method.In the mean time,the energy dispersive X Ray microanalysis method was used to measure the intracellular Ca ++ concentration.The changes of intracellular Ca ++ concentration at the end of circulatory arrest with addition of fructose 1 6 diphosphate (FDP) cerebroplegia were measured by means of EDS as well.The results indicated that the ultrastructure of brain neuron was damaged severely,and intracellular Ca ++ concentration increased significantly after DHCA 120 minutes.But using FDP cerebroplegia during DHCA could alleviate intracellular calcium accumulating and reduce brain damages.It may be a useful pharmacological method for the brain protection under DHCA. 