乙肝患者血清中HBeAg与乙肝病毒DNA关系的探讨

The relationship between serum HBeAg and HBV-DNA in the patients infected hepatitis B virus

目的:探讨乙肝患者血清中e抗原(HBeAg)与乙肝病毒DNA(HBV-DNA)之间的关系。方法:选取305例HBsAg阳性的乙肝患者血清标本,分别用微粒子酶免疫分析法(MEIA)和核酸扩增荧光定量法(FQ-PCR)检测HBeAg和HBV-DNA。结果:305例标本中,HBeAg阳性率为71.48%,而HBV-DNA阳性率为58.69%。HBeAg阳性标本中,HBV-DNA阳性率为66.05%;而HBeAg阴性标本中,HBV-DNA阳性率为40.23%,两者有显著性差异(χ2=17.11,P<0.01)。不同浓度组HBeAg和HBV-DNA检测结果比较,有统计学意义(χ2=35.67,P<0.01)。结论:HBV-DNA比HBeAg能更准确反映出乙肝病毒复制情况,可以更好地指导临床进行乙肝治疗。

Objective：To explore the relationship between serum HBeAg and HBV-DNA contents. Methods：305 serum samples which have shown HBsAg positive were included.HBeAg was tested by microparticle enzyme immunoassays （MEIA）and HBV-DNA was tested by fluorescence quantitative polymerase chain reaction （FQ-PCR）. Results： 71.48% of 305 samples showed HBeAg positive, while 58.69% samples showed HBV-DNA positive.In the HBeAg positive samples, the positive percentage of HBV-DNA was 66.05%,and in the HBeAg negative samples,the positive percentage of HBV-DNA was 40.23%,which showed there was significant difference（Х^2=17.11 ,P〈0.01）between HBeAg and HBV-DNA.The differences between HBeAg in a series of concentration and the test results of HBV-DNA were also significant（Х^2=35.67 ,P〈 0.01 ）. Conclusion： Compared with HBeAg,HBV-DNA is a better maker for detecting the replication of HBV （hepatitis B virus）,which would be helpful for the HBV clinical therapy.