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酵母表面展示技术应用的研究进展 被引量:5

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摘要 酵母菌的蛋白表达方式与高等真核生物细胞十分相似,酵母表面展示技术是研究真核生物,尤其是人类蛋白质表达的理想方法之一。酵母菌表面标记可溶性配基,通过流式细胞技术对酵母菌展示文库进行快速、精确的筛选,其已广泛用于研究天然蛋白质-蛋白质的相互作用、抗体Fab片段的亲和力,成熟、抗体的检测与筛选,以及功能蛋白质抗原表位图谱绘制等。
作者 翟一凡 邓宁
出处 《生物学通报》 北大核心 2009年第2期4-6,共3页 Bulletin of Biology
基金 广州市科技公关计划(2006Z2-E4051)
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参考文献12

  • 1贾帅争.真核蛋白表面展示系统[J].国外医学(分子生物学分册),2000,22(1):49-52. 被引量:4
  • 2Boder E.T., Wittrup K.D.. Yeast surface display for screening combinatorial polypeptide libraries. Nat Biotechnol,1997,15(6): 553-557.
  • 3郭波,谢佩蓉,邹强,郑萍.酵母表面展示系统研究进展[J].生物化学与生物物理进展,2002,29(1):19-22. 被引量:18
  • 4Feldhaus M.,Siegel R..Flow cytometric screening of yeast surface display libraries.Methods Mol Biol,2004,263:311--332.
  • 5Lydia Blaise,Anita Wehnert,Mieke P.G..Steukers et al.Construction and diversification of yeast cell surface displayed libraries by yeast mating:application to the affinity maturation of Fab antibody fragments.Gene,2004,342:211--218.
  • 6Consuelo Garcia-Rodriguez,Raphael Levy,Joseph W. Amdt et al.Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxin. Nature Biotechology, 2007,25:107 -- 116.
  • 7Chao G.,Coehran J.R.,Dane Wittrup K..Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display.J Mol Biol,2004, 342:539--550.
  • 8Wadle A.,Kubuschok B.,Imig J.et al.Serological immune response to cancer testis antigens in patients with pancreatic cancer.Int J Cancer,2006,119:117-- 125.
  • 9Xin Xiang Wang,Yong Ku Cho, Eric V.Shusta. Mining a yeast library for brain endothelial cell-binding antibodies. Nature Methods,2007,(4): 143-- 145.
  • 10唐语谦,叶茂,林影,韩双艳,郑泓,王小宁,梁世中.人源蛋白酶体α亚基6(Proteasome subunit alpha 6)在酿酒酵母表面展示[J].生物化学与生物物理进展,2007,34(9):984-990. 被引量:4

二级参考文献54

  • 1肖朝庭,傅衍,田勇.酿酒酵母细胞表面工程应用研究新进展[J].微生物学报,2005,45(5):812-816. 被引量:5
  • 2范薇,于长明,杨敬,隋丽华,战大伟,贺争鸣,孙岩松.重组钩端螺旋体外膜蛋白酶联免疫吸附(ELISA)检测方法的建立[J].中国实验动物学报,2005,13(4):249-252. 被引量:2
  • 3[1]Moriki T, Kuwabara I, Liu F T, et al. Protein domain mapping by lambda phage display: the minimal lactose binding domain of galectin-3. Biochem Biophys Res Commun, 1999, 265(2): 291~296
  • 4[2]Efimov V P, Nepluev I V, Mesyanzhinov V V. Bacteriophage T4 as a surface display vector. Virus Genes, 1995, 10(2): 173~177
  • 5[3]Lattemann C T, Maurer J, Gerland E, et al. Autodisplay: functional display of active beta-lactamase on the surface of Escherichia coli by the AIDA-I autotransporter. J Bacteriol, 2000, 182(13): 3726~3733
  • 6[4]Ernst W J, Spenger A, Toellner L, et al. Expanding baculovirus surface display: Modification of the native coat protein gp64 of Autographa californica NPV. Eur J Biochem, 2000, 267(13): 4033~4039
  • 7[5]Boder E T, Wittrup K D. Yeast surface display for screening combinatorial polypeptide libraries. Nat Biotechnol, 1997, 15(6): 553~557
  • 8[6]de Nobel J G, Klis F M, Priem J, et al. The glucanase-soluble mannoproteins limit cell wall porosity in Saccharomyces cerevisiae. Yeast, 1990, 6(6): 491~499
  • 9[7]Lu C F, Montijn R C, Brown J L, et al. Glycosyl hosphatidylinositol dependent cross-linking of alpha-agglutinin and beta 1,6-glucan in the Saccharomyces cerevisiae cell wall. J Cell Biol, 1995, 128(3): 333~340
  • 10[8]Schreuder M P, Brekelmans S, van den Ende H, et al. Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae. Yeast, 1993, 9(4): 399~409

共引文献22

同被引文献46

  • 1贾艳萍,魏群,赵军.对酵母细胞酶法破壁的研究[J].中国酿造,2005,24(9):11-13. 被引量:33
  • 2CLAY T M,CUSTER M C,SACHS J,et al.Efficient transfer of a tumor antigen-reactive TCR to human peripheral blood lymphocytes confers anti-tumor reactivity[J].J Immunol,1999,163 (1):507 -513.
  • 3COOPER L J,KALOS M,LEWINSOHN D A,et al.Transfer of specificity for human immunodeficiency virus type 1 into primary human T lymphocytes by introduction of T-cell receptor genes[J].J Virol,2000,74(17):8207 -8212.
  • 4XUE S A,GAO L,HART D,et al.Elimination of human leukemia cells in NOD/SCID mice by WTI-TCR genetransduced human T cells[J].Blood,2005,106(9):3062-3067.
  • 5MORGAN R A,DUDLEY M E,WUNDERLICH J R,et al.Cancer regression in patients after transfer of genetically engineered lymphocytes[J].Science,2006,314(5796):126 -129.
  • 6RICHMAN S A,HEALAN S J,WEBER K S,et al.Development of a novel strategy for engineering highaffinity proteins by yeast display[J].Protein Eng Des Sel,2006,19(6):255-264.
  • 7FUKUDA T,ISHIKAWA T,OGAWA M,et al.Enhancement of cellulase activity by clones selected from the combinatorial library of the cellulose-binding domain by cell surface engineering[J].Biotechnol Prog,2006,22(4):933 -938.
  • 8FUKUDA N,ISHII J,SHIBASAKI S,et al.High-efficiency recovery of target cells using improved yeast display system for detection of protein-protein interactions[J].Appl Microbiol Biotechnol,2007,76(1):151-158.
  • 9LIN Y,SHIRAGA S,TSUMURAYA T,et al.Isolation of novel catalytic antibody clones from combinatorial library displayed on yeast-cell surface[J].J Mol Catal B:Enzym,2004,28(4-6):247 -251.
  • 10WANG X X,CHO Y K,SHUSTA E V.Mining a yeast library for brain endothelial cell-binding antibodies[J].Nat Methods,2007,4(2):143-145.

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