两种石蒜中酯酶和苹果酸脱氢酶酶谱分析

Analysis of Esterase and Malate Dehydrogenase on Two Different Amaryllis

对2种石蒜用3种方法提取酶液,采用聚丙烯酰胺凝胶电泳进行酯酶和苹果酸脱氢酶同工酶酶谱分析,同时对提取酶液在白色点滴板上显色。结果表明:从酶谱分析可知,石蒜酯酶有1条谱带,相对迁移率(Rf)为0.17;苹果酸脱氢酶有2条谱带,Rf值分别为0.51和0.69。忽地笑酯酶有1条谱带,Rf值为0.17;苹果酸脱氢酶有4条谱带,Rf值分别为0.43,0.47,0.51和0.69。从显色试验可知,3种提取方法对酶提取的充分性、活性有差异;研磨后,经60%超声的提取方法对酶能够充分提取,而且能更好地保持酶的活性。

In this paper, two kinds of Amaryllis were analyzed based on FAST and MDH by polyacrylamide gel electropho-resis（PAGE） and enzyme reaction color test. The results indicated that the L. aurea had one EST band, the relative mobility rates（Rf） was 0.17, two MDH bands, Rf 0.51 and 0.69 respectively. The L. radidta had a same EST band, four MDH bands, Rf 0.43, 0.47, 0.51 and 0.69 respectively. Color test showed that three extraction methods had differences; the extraction method of 60% ultrasound after rubbing was the best for extracting the enzyme adequately and activity preferably.