抗寒苹果“新冠”的茎尖组织培养

Shoot-Tip Tissue Culture of Cold-Resistant Malus Xinguan

以苹果"新冠"茎尖为材料,通过研究不同的激素配比对芽增殖和生根的影响,探索抗寒苹果"新冠"的最适增殖培养基和生根培养基。结果表明:"新冠"的最适诱导培养基为MS+0.1 mg/L NAA+1.5 mg/L 6-BA;最适增殖培养基为MS+1.0 mg/L 6-BA+0.05或0.08 mg/LNAA,其增殖系数达到7.4;最适生根培养基为1/2MS+0.2 mg/L IBA+0.6 mg/L IAA,其生根率达到90.5%。

The effect of different phytohormone ration on the multiplication and rooting of shoot-tip explants from Malus Xinguan were studied. The results indicated that the most suitable induced medium was MS＋0.1 mg/L NAA＋1.5 mg/L 6-BA; the most suitable multiplication medium was MS＋1.0 mg/L 6-BA＋0.05 or 0.08 mg/L NAA, the multiplication index was 7.4; the best rooting media was 1/2MS＋0.2 mg/L IBA＋0.6 mg/L IAA, the rooting rate was 90.5%.