摘要
SYBR GreenⅠ是一种特异结合双链DNA的染料,已被证明能灵敏地检测核酸。采用SYBRGreenⅠ荧光PCR熔解曲线,通过摸索、优化PCR反应条件及反应体系,扩增出两条不同大小、Tm值不同的目的核酸片段,表现在熔解曲线上出现两个独立的波峰,建立了用SYBR GreenⅠ荧光PCR熔解曲线来检测多个基因的方法,从而实现不用核酸探针,简单、经济、快速的多重荧光PCR方法检测沙门菌和产单核细胞李斯特菌。
SYBR Green Ⅰ is a special dye that can combine with double-stranded DNA. It is proved that the dye can sensitively inspect nucleic acid. Based on the intercalating dye and optimizing the reaction conditions and reaction system,analyzing of the melting curves of the amplified products, two different length and different Tin-value nucleic acid fragments were amplified. There are two separate wave crests in the melting curves. This method can inspect many genes based on the dye, not based on sequence-specific probes, and offer an economic, simple, fast real-time PCR system to inspect Salmonella and Listeria monocytogenes.
出处
《动物医学进展》
CSCD
北大核心
2009年第2期12-15,共4页
Progress In Veterinary Medicine
基金
深圳出入境检验检疫局科技项目(2006SZK01)
