微波碱法提取小麦胚蛋白工艺条件的探讨

Studies on Technological Conditions for Extracting Wheat Germ Protein by Microwave-Alkali Method

应用微波加热碱提取技术对小麦胚进行蛋白质提取,通过单因素实验和正交实验得到了微波碱法提取小麦胚蛋白的最佳工艺条件,即粉碎粒度30目,碱料比为4∶1,微波时间120s,微波温度为高火,碱提时间40min,碱提温度45℃,碱提浓度0.1mol/L,此条件下得到的小麦胚蛋白的提取率为71.07%。

The best technological conditions of extracting wheat germ protein through microwave heating technology were obtained through single factor experiment and orthogonal experiment, namely reduction ratio 30 mesh, alkali material ratio 4：1, microwave time 120 s, microwave temperature high fire, alkali extraction time 40 min, alkali extraction temperature 45℃, alkali extraction concentration 0.1 mol/1. Whereby the extraction rate of wheat germ protein obtained was 71.07%.