补充谷氨酰胺对过度训练大鼠肾脏滤过屏障形态结构和尿液成分的影响

Effects of Glutamine and Soybean Peptide Supplementation on the Structure of Kidney Filtration Barrier and Component of Urinary of Over-training Rats

目的探讨补充谷氨酰胺对过度训练大鼠肾脏滤过屏障形态结构和功能的影响。方法将30只雄性大鼠随机平均分为3组,分别为对照组(C)、过度训练组(OT)、过度训练+补充谷氨酰胺组(OG)。检测各组大鼠尿液总蛋白、白蛋白和β-微球蛋白含量和观察各大鼠肾脏滤过屏障形态结构的变化。结果电镜下,过度训练组大鼠肾组织出现毛细血管球系膜增生,基膜基质增多,足细胞肿胀、融合,裂孔不清晰等变化;OG组大鼠肾小球基底膜厚度均匀一致;足细胞排列有序,偶见足细胞足突有轻度融合现象,裂孔大小均匀一致。与C组相比,OT组大鼠血清睾酮水平显著降低,皮质醇水平显著增高,尿液中的TP、Alb、β2-MG含量显著增高;与OT组相比,OG组TP、Alb、β2-MG含量显著降低。结论补充谷氨酰胺对过度训练大鼠的肾脏滤过屏障形态结构有维持作用,降低了过度训练大鼠尿液TP、Alb、β2-MG含量,在一定程度上抑制了运动性尿蛋白的发生。

Objective To investigate the effects of glutamine supplementation on the structure of kidney filtration barrier and component of urinary of over - training rats. Methods 30 male rats were randomly divided into four groups, named as the control group （C）, over - training group （OT）, and over - training ＋ glutamine group （OG）. Examine the content of TP, Alb , β2 - MG and observe the changes of filtration barrier in each group. Results Under the electron microscope, the OT group appeared glomerulus mesangium hyperplasia, glomerular basement membrane increased, podocyte swelled, inosculate, and the slit pore was not clearly. The thickness of glomerular basement membrane symmetrical, the array of podocyte orderly, the foot process appeared light amalgamation occasionally. The slit pore was symmetrical of OG group. Compared with C, OT rats＇ serum testosterone levels significantly decreased, corticosterone levels were significantly increased at the same time. The content of TP,Alb,β2- MG in urine incrcased roughly. Compared with OT group, the content of TP,Alb,β2 - MG in urine descreased significantly. Conclusion The supplement of glutamine and soybean peptides had important effects on supporting kidney filtration barrier and lowered the content of TP,AIb,β2- MG over- training rats＇urine. Inhabited exercise induced urinary protein in a certain extent.