应用组织芯片技术构建细胞免疫芯片

Construct cell immuno-chip with tissue microarray technique

目的探讨应用组织芯片制作技术构建细胞免疫芯片的方法。方法设计布有多形性胶质母细胞瘤、人脑胶质瘤SHG-44细胞、神经干细胞、裸鼠骨髓细胞共24例的细胞微阵列,应用组织芯片仪制作细胞芯片蜡块,切片后进行CDC2的"EnVision"法免疫组化染色。结果研制的24位点细胞芯片,细胞排列整齐,免疫组化染色效果好。结论应用组织芯片制作技术构建细胞免疫芯片简单易行,结果可靠,开创了一种制作细胞免疫芯片的新方法。

[Objective] To explore feasibility of constructing cell immuno-chip with tissue microarray technique. [Methods] To design cell microarrays containing 24 specimens, including glioblastoma muhiforme ceils, SHG-44 cells, neural stem cells and bone marrows of nude mice. A tissue mieroarray instrument was used to prepare these cell microarray paraffin blocks, which were made into slices and immunohistochemistry staining for CDC2 by ＂EnVision＂ method. [Results] The dots of cell microarrays arranged orderly, its morphology were preserved well and effect of immunohistochemistrical staining were reliable. [Conclusion] Using tissue mieroarray technique to fabricate cell immuno-chip is simple, reliable and cost-effective, which innovates a novel technology for establishing cell immuno-cbip.