儿童传染性单核细胞增多症患者GST基因遗传多态性的研究

Genetic polymorphism of glutathione s-transferase associated with childhood infectious mononucleosis

目的探讨谷胱甘肽S-转移酶T1(GSTT1)和M1(GSTM1)基因多态性与儿童传染性单核细胞增多症(IM)发病风险的关系。方法采用病例-对照研究方法对52例IM患儿和60名健康儿童进行多重PCR技术检测GSTT1和GSTM1基因多态性,分析不同基因型与儿童IM发病的关系。结果IM组和对照组GSTT1纯合缺失基因型[GSTT1(-/-)]的分布频率分别为59.62%和43.33%,组间比较差异有显著性(χ2=7.231,P<0.05);携带GSTT1(-/-)基因型儿童发生IM的风险是携带GSTT1非纯合缺失基因型[GSTT1(+/+)或(+/-)]儿童的1.931倍[比值比(OR)=1.931,95%可信区间(CI):1.038～2.894]。GSTM1纯合缺失基因型[GSTM1(-/-)]的分布频率在IM组和对照组间差异无显著性(P>0.05)。基因型联合分析显示:携带GSTT1(-/-)与GSTM1(-/-)联合基因型的儿童,其IM的发病风险显著增加(OR=2.691,95%CI:0.902～6.127)。结论GSTT1纯合缺失基因型可能是儿童IM发生的重要危险内因之一。

[Objective] To investigate the association of glutathione S-transferase T1 （GSTrl） and glutathione Stransferase M1 （GSTM1） gene polymorphism with childhood infectious mononucleosis （IM）. [Methods] The casecontrol study was conducted involving 52 childhood IM patients and 60 controls. The genetic polymorphisms of GSTI1 and GSTM1 were detected with multiplex polymerase chain reaction （PCR）. The distribution difference of the genotypes between them was analyzed. [Results] The frequencies of GSTT1 null genotype was 59.62%, 43.33% in childhood IM and controls （Х^2=7.231, P〈0.05）, respectively. There were 1.931 times of risks with occurrence IM for childhood individuals who carrying GSTrl null genotype than those carrying GSTT1 non-null genotype （OR=1.931, 95%GI： 1.038-2.894）. There was no significant difference in the frequencies of GSTM1 null genotype in childhood IM and controls. Childhood individuals carrying both GSTT1 and GSTM1 null genotype were at high risks of occurrence IM （OR=2.691, 95%CI： 0.902-6.127）. [Conclusion] The results of this study suggest that GSTT1 null genotype plays an important role in the occurrence of childhood IM.