MAPK在前列腺癌和增生组织中的表达及其意义

Expression of MAPKs in benign prostatic hyperplasia prostatic cancer and its clinical significance

目的通过丝裂素激活蛋白激酶(mitogen activated protein kinases,MAPK)在前列腺癌(prostaticcancer,PC)、前列腺增生症(benign prostatic hyperplasia,BPH)及正常前列腺中的表达,探讨MAPK在PC和BPH形成过程中的作用。方法采用免疫组织化学S-P法对40例BPH、15例PC、10例正常前列腺患者标本进行检测。结果ERK在BPH、PC的上皮细胞和基质细胞胞浆、胞核中均有着色,正常前列腺基质细胞胞浆、胞核均着色,上皮细胞无核着色。ERK在BPH基质细胞核染色约为79%,而PC约为50%,正常前列腺48%。前者与后两者差异存在显著性,(P≤0.05)。而PC和正常前列腺两者间差异无显著性(P>0.05)。BPH上皮细胞核染色约为72%,PC上皮细胞核染色为0。三者间均存在着明显差异,均具有显著性(P≤0.05)。JNK在BPH、PC及正常前列腺上皮细胞及基质细胞中均染色,但核染色较低,而且他们之间差异无显著性(P>0.05)。P38在前列腺正常组织和增生组织均有表达,两者差异无显著性。在PC中上皮细胞增加,基质细胞表达明显减少。与正常前列腺表达差异存在显著性(P≤0.05)。结论ERK的过度激活,是BPH和PC发生的一条主要信号传递途径。JNK与BPH和PC的发生的病理机制无关。P38的相对抑制是BPH和PC发生的病理机制的另一个方面。

[Objective] To investigate the expression of mitogen activated protein kinases （MAPKs） in benign prostatic hyperplasia （BPH）, prostatic cancer （PC） and normal prostatic tissue and explore the molecular biologymechanism of pathogenesis of BPH. [Methods] To detect the expression of extracellular signal regulated kinase （ERK）, c2Jun N2 terminal kinase （JNK） and p38 MAPK in 40 cases of BPH, 15 cases of PC and 10 cases of normal prostates with immuno histochemical techniques. [Results] ERK is expressed in both plasma and nuleus of metrical and epidermal ceils in BPH and PC with different degrees. ERK is expressed in both plasma and nuleus only of metrical cells, while in epidermal cells, it＇s only in plasma. Positive ratio of ERK in metrical cells nucleus exists significant difference between BPH and the latter two （P ≤0.05）, while there is no difference between PC and normal prostate tissue （P 〉0.05）. Positive ratio of ERK in epidermal cell nucleus is significant difference among those three kinds of tissue （P ≤0.05）. JNK is expressed in both plasma and nuleus of metrical and epidermal cell in BPH, PC and normal tissue, but the expression in nucleus is low. There exists no significant difference among them. Expressions of P38 in epidermal ceils in normal prostate and BPH tissue is no difference Expressions of P38 in epidermal cells in PC increased considerably, and it＇s about 57%, while in metrical cells, it＇ s only about 15%. There is significant difference between PC and normal tissue （P ≤0.05）. [Conclusion] The over expression of ERK leading to the increase of proliferative index of cells and down expressions of p38 MAPK leading to the decrease of apoptosis may be involved in the development of BPH and PC.