粉防己碱对人宫颈癌Hela细胞增殖与凋亡的影响

Effect of tetrandrine on proliferation and apoptosis of human cervical cancer cell line hela

目的观察粉防己碱对人宫颈癌Hela细胞增殖与凋亡的影响,探讨粉防己碱对宫颈癌的体外抗肿瘤效应。方法采用MTT比色法观察粉防己碱对Hela细胞的增殖抑制效应,利用细胞DNA琼脂糖凝胶电泳及流式细胞术检测粉防己碱诱导肿瘤细胞凋亡的作用,以免疫细胞化学法检测粉防己碱对Bcl-2和Bax表达水平的影响。结果粉防己碱对Hela细胞增殖有抑制作用,其抑制效应具有剂量依赖的特点。粉防己碱可诱导Hela细胞凋亡,可上调Bax基因表达,下调Bcl-2基因表达。结论粉防己碱对Hela细胞增殖的抑制效应具有剂量依赖性,并可诱导细胞凋亡,其抗肿瘤效应可能与凋亡相关基因表达的调控有关。

[Objective] To investigate the anti-tumor effect of tetrandrine on human cervical cancer cell line Hela in vitro. Effects of tetrandrine on proliferation and apoptosis of human ovarian cancer cell Hela were observed. [Methods] The effects of tetrandrine on proliferation of Hela cells was observed by MTT assay and clonogenic assay. Apoptosis was observed by acridine orange （AO）/ethidium bromide （EB） Fluorescent staining, DNA gel electrophoresis and flow cytometry, and the expression of apoptosis related gene was analyzed by immunohistochemical staining method. [Result] Tetrandrine inhibits the proliferation of Hela cells in a dosage dependent manner and induces apoptosis. Immunohistochemical staining showed that the expression of Bcl-2, decreasing and the expression of Bax increasing in Hela cells treated with tetrandrine. [Conclusion] Tetrandrine inhibits the proliferation of Hela in the dose dependent manner, and induces apoptosis. The antitumor effect of tetrandrine may be due to the regulation of the expressions of Bcl-2 and Bax.