肾血管性高血压大鼠实验模型简易制作方法的比较研究

The Comparative Study on How-to-make Experimental Rat Models of Renovascular Hypertension

目的:探讨中医药治疗高血压的实验研究中肾性高血压大鼠实验模型的简易制作方法。方法:严格筛选w istar大鼠36只,随机分成3组,阴性对照组、模型对照组和模型组各12只。阴性对照组仅游离肾动脉,不予缩窄;模型对照组采用传统银夹狭窄左肾动脉;模型组取针灸银针紧贴左肾动脉,用手术丝线将二者一起捆扎,抽去银针,制作左肾动脉血流部分被阻断模型。比较各组大鼠血压变化参数。结果:术后第8周,模型对照组与模型组比阴性对照组显著提高大鼠血压参数(t=18.0640、21.9070,P<0.001),且模型组血压参数高于模型对照组(t=2.8935,P<0.05)。结论:该简易方法缩窄肾动脉形成肾性高血压大鼠模型完全达到并且优于传统两肾一夹高血压大鼠模型的标准,此方法与传统制作方法比较具有操作简单、手术成功率高、可控制性强、模型稳定等特点。

Objective： To approach how to make experimental rat models of renovascular hypertension in TCM empirical study of treating hypertension. Method ： 36 rats selected strictly were divided into 3 groups randomly, which were negative control group, model control group and model group. Each group had 12 rats. Renal arteriae of rat in negative control group was dissociated but not coarctated. The one in model control group was coarctated with traditional silver clip. And the one in model group was done as follows： picking up left renal artery with acu - needle, tying up them with suture silk, and take away the needle then to be the model of obstructed left renal artery. Compare the data of blood pressure in each group. Result： After 8 weeks of operation, the data of blood pressure in model control group and model group were increased more obviously than in negative control group （ t = 18.064 0,21. 907 0,P 〈0. 001 ） , and those in model group were higher than in model control group （t =2. 893 5, P 〈 0. 05）. Conclusion ： Compared with traditional methods, the above-method has the characteristics of simple technique, high successful operation, strong operability and steady models. It also proved the produce method of renovascular hypertension rats completely reached the standards of 2K1C hypertension rat model, and provide the experimental basis for researching and studying experimental hypertension model.