期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Distribution of an Ankyrin-repeat Protein on the Endoplasmic Reticulum in Arabidopsis

Distribution of an Ankyrin-repeat Protein on the Endoplasmic Reticulum in Arabidopsis
原文传递
导出
摘要 There are many ankyrin-repeat proteins in plant cells. However, the distribution and function of these proteins are mostly unclear. By reverse transcription-polymerase chain reaction, a gene encoding an ankyrin-like protein was cloned from Arabidopsis and named AtANK1 （GenBank accession no. NM_120340）. The 6-His-tagged AtAnkl-N fusion protein was affinity-purified and its rabbit polyclonal antibody was obtained. Immuno-blotting with the purified anti-AtAnkl-N polyclonal antibody revealed that the relative molecular weight of the AtANK1 protein was about 76 kDa. By immunofluorescence labeling and immuno-gold labeling with the purified anti-AtAnkl-N polyclonal antibody, coupled with confocal and transmission electron microscopy observation. AtANK1 was found to be distributed on the membrane of the endoplasmic reticulum in Arabidopsis cells. Based on these results, we suggested that AtANK1 might be involved in endoplasmic reticulum-related protein localization and sorting in plant cells. There are many ankyrin-repeat proteins in plant cells. However, the distribution and function of these proteins are mostly unclear. By reverse transcription-polymerase chain reaction, a gene encoding an ankyrin-like protein was cloned from Arabidopsis and named AtANK1 （GenBank accession no. NM_120340）. The 6-His-tagged AtAnkl-N fusion protein was affinity-purified and its rabbit polyclonal antibody was obtained. Immuno-blotting with the purified anti-AtAnkl-N polyclonal antibody revealed that the relative molecular weight of the AtANK1 protein was about 76 kDa. By immunofluorescence labeling and immuno-gold labeling with the purified anti-AtAnkl-N polyclonal antibody, coupled with confocal and transmission electron microscopy observation. AtANK1 was found to be distributed on the membrane of the endoplasmic reticulum in Arabidopsis cells. Based on these results, we suggested that AtANK1 might be involved in endoplasmic reticulum-related protein localization and sorting in plant cells.
作者 LiqinWei Yan Li
机构地区 State Key Laboratory of Plant Physiology and Biochemistry
出处 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2009年第2期140-146,共7页 植物学报（英文版）
基金 Supported by the National Natural Science Foundation of China (30570924 and 30721062)
关键词 ankyrin-repeat protein ARABIDOPSIS endoplasmic reticulum subcellular localization western blotting. ankyrin-repeat protein Arabidopsis endoplasmic reticulum subcellular localization western blotting.
分类号 Q943.1 [生物学—植物学]
  • 相关文献

参考文献29

  • 1Albert S, Despres B, Guilleminot J, Bechtold N, Pelletier G, Delseny Met al. (1999). The EMB 506 gene encodes a novel ankyrin repeat containing protein that is essential for the normal development of Arabidopsis embryos. Plant J. 17, 169-179.
  • 2Becerra C, Jahrmann T, Puigdomenech P, Vicient CM (2004). Ankyrin repeat-containing proteins in Arabidopsis: characterization of a Hovel and abundant group of genes coding ankyrin-transmembrane proteins. Gene 340, 111-121.
  • 3Bennett V, Chen L (2001). Ankyrins and cellular targeting of diverse membrane proteins to physiological sites. Curt. Opin. Cell Biol. 13, 61-67.
  • 4Bennett V (1992). Ankyrins: adaptors between diverse plasma membrane proteins and the cytoplasm. J. Biol. Chem. 267, 8703-8706.
  • 5Boevink P, Oparka K, Cruz SS, Martin B, Betteridge A, Hawes C (1998). Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network. PlantJ. 15.41-47.
  • 6Cao H, Glazebrook J, Clarke JD, Volko S, Dong X (1997). The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell 88, 57-63.
  • 7Chinchilla D, Merchan F, Megias M, Kondorosi A, Sousa C, Crespi M (2003). Ankyrin protein kinase: a novel type of plant kinase gene whose expression is induced by osmotic stress in alfalfa. Plant Mel. Biol. 51. 555-566.
  • 8Devarajan P, Stabach PR, Mann AS, Ardito T, Kashgarian M, Morrow JS (1996). Identification of a small cytoplasmic ankyrin (AnkG119) in kidney and muscle that bindsβ/∑* spectrin and associates with the Golgi apparatus. J. Cell Biol, 133, 819-830.
  • 9Holzinger A, De Ruijter N, Emons AM, LuTz-Meindl U (1999). Spectrin-like protein in green algae (Desmidiaceae). Cell Biol. Inter. 23, 335-344.
  • 10Jonas-Straube E, Hutin C, Hoffman NE, Schunemann D (2001). Functional analysis of the protein-interacting domains of chloroplast SRP43. J. Biol. Chem. 276, 24654-24660.
Journal of Integrative Plant Biology
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部