银杏叶提取物对糖尿病大鼠血清学指标的影响

Effect of Ginkgo biloba Extract on Serological Indexes of Diabetic Rats

目的研究银杏叶提取物(GbE)对糖尿病大鼠血清学指标的影响,探讨其在糖尿病大鼠脂代谢过程中的作用。方法通过腹腔注射链脲佐菌素(STZ)(50mg/kg)建立糖尿病大鼠模型。将模型大鼠随机分为模型对照组(DM)和GbE组,GbE组每日给予GbE提取物,0.83ml/只,DM组每日给予等剂量的生理盐水,均为腹腔注射,另设1组正常大鼠对照。于注射银杏叶提取物的第1和15天采血,检测各组大鼠血清甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平,第15天检测大鼠血清中胰岛素和C-肽水平。结果DM组大鼠血清中TG和LDL-C水平随着时间的延长逐渐升高;注射后第15天,GbE组大鼠血清中TG和LDL-C水平均显著低于DM组,HDL-C和C-肽水平显著高于DM组,胰岛素水平与DM组比较,差异无统计学意义。结论银杏叶提取物对糖尿病大鼠的脂代谢具有调节作用,对高糖导致的损伤具有保护作用。

Objective To study the effect of Ginkgo biloba extract （GbE） on serological indexes as well as its role in lipid nletabolism of diabetic rats. Methods Rat model of diabetes was established by injecting i.p. with streptozotocin （STZ）. The diabetic rats were divided into GbE and model control （DM） groups and daily injected, i. p. with 0. 83 ml of GbE and physiological saline respectively for 14 d. Healthy rats were served as normal control. The serum samples of rats in various groups were collected on days 1 and 15 after the first injection for determination of triglyceride （TG）, high density lipoprotein （HDL-C） and low density lipoprotein （LDL-C）. The insulin and C-peptide levels in sera of rats were determined on day 15 after the first injection. Results The TG level in sera of rats in DM group increased gradually as the time goes on. On day 15 after the first injection, the TG and LDL-C levels in sera of rats in GbE group were significantly lower, while HDL-C and C-peptide levels were significantly higher than those in DM group. However, no significant difference was observed in the insulin levels of the two groups. Conclusion GbE showed regulatory effect on lipid metabolism of diabetic rats and protective effect on injury caused by high blood suger.