不同浓度四君子汤对尾部悬吊大鼠比目鱼肌肌萎缩的影响

Effects of Different Doses of Sijunzi Decoction on Soleus Atrophy in Tail-suspended Rats.

目的观察不同浓度四君子汤对尾部悬吊大鼠比目鱼肌肌萎缩的影响。方法尾部悬吊法建立模拟失重模型,30只Sprague-Dawley大鼠随机分为5组,同步饲养组(CON)、尾吊组(HLU-W)、尾吊+四君子汤高(HLU-H)、中(HLU-M)、低(HLU-L)浓度组,每组6只。用免疫组织化学法观察药物对比目鱼肌肌球蛋白重链(MHC)的影响。结果与尾吊组相比,四君子汤高、中浓度组使比目鱼肌湿重分别增加了33.11%和37.57%,与同步饲养组亦无显著性差异;四君子汤高、中、低浓度组使比目鱼肌Ⅰ型肌纤维比例增加了34.04%～54.90%,Ⅰ型肌横截面积增加了8.50%～36.86%,且中浓度组与同步饲养组相比亦无显著性差异。结论3种浓度的四君子汤对模拟失重引起的比目鱼肌肌萎缩有显著的对抗作用,且中浓度四君子汤的效果优于高、低浓度。

Objective To observe the effects of different doses of Sijunzi decoction （SJZD） on soleus（SOL） atrophy in tail-suspended rats. Methods Weightlessness was simulated by tail suspension in female Sprague- Dawley rats. Six rats in each group were assigned to a 5-group design randomly： control （CON） ; hind limb unloading （HLU） plus intragastric water instillation （HLU-W）, as well as plus intragastric high dose（ HLU- H）, middle dose （HLU-M） and low dose （HLU-L） SJZD instillation respectively. Immunohistochemical method was used to observe the effects of them on expression of myosin heavy chain （MHC） isoforms in soleus muscle. Results Compared with HLU-W, muscle wet weight （MWW） of SOL in high and middle dose group increased by 33.11% and 37.57% respectively, as well as no significant difference with CON; three doses of SJZD groups increased the percentage of type I myosin by 34.04% - 54.90% and its cross sectional area （CSA） by 8.50% -36.86%, moreover SJZD of middle dose group showed no significant difference with that of CON group. Conclusion Three doses of SJZD groups can significantly prevent soleus atrophy induced by simulated weightlessness, and middle dose is the more effective one than high and low dose.