一种仿生型心肌组织工程生物反应器的设计

Design on Bionic Myocardial Tissue Engineering Bioreactor.

目的研制一种仿生型心肌组织工程生物反应器。方法根据心肌细胞培养的要求,设计了温度、pH值、CO2浓度、溶解氧含量参数检测和控制电路及细胞拉伸加载装置及电场刺激装置。结果实验结果表明,该生物反应器能够准确检测各项参数,并给细胞合适的拉伸频率和幅度及电场刺激。结论研制的生物反应器能够在一定程度上模拟人体心肌工作环境。

Objective To develope a kind of bionic myocardial tissue bioreactor. Methods According to the requirements of cardiac cell cultivation, several circuits for measuring and controlling parameters such as temperature, pH value, CO2 concentration,O2 saturation degree, cell stretched equipment and electrical stimulating device were designed. Results The bioreactor was able to measure all parameters, and provide electrical stimulation and stretch load to cells with proper strain and frequency. Conclusion This developed bioreactor can simulate internal working circumstance of human myocardial tissue in a certain degree.